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Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues

The expression pattern of Sohlh1 (spermatogenesis and oogenesis specific basic helix-loop-helix 1) and Sohlh2 in mice has been reported in previous studies. Sohlh1 and Sohlh2 are specifically expressed in spermatogonia, prespermatogonia in male mice and oocytes of primordial and primary follicles in...

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Autores principales: Zhang, Xiaoli, Liu, Ruihua, Su, Zhongxue, Zhang, Yuecun, Zhang, Wenfang, Liu, Xinyu, Wang, Fuwu, Guo, Yuji, Li, Chuangang, Hao, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574203/
https://www.ncbi.nlm.nih.gov/pubmed/26375665
http://dx.doi.org/10.1371/journal.pone.0137431
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author Zhang, Xiaoli
Liu, Ruihua
Su, Zhongxue
Zhang, Yuecun
Zhang, Wenfang
Liu, Xinyu
Wang, Fuwu
Guo, Yuji
Li, Chuangang
Hao, Jing
author_facet Zhang, Xiaoli
Liu, Ruihua
Su, Zhongxue
Zhang, Yuecun
Zhang, Wenfang
Liu, Xinyu
Wang, Fuwu
Guo, Yuji
Li, Chuangang
Hao, Jing
author_sort Zhang, Xiaoli
collection PubMed
description The expression pattern of Sohlh1 (spermatogenesis and oogenesis specific basic helix-loop-helix 1) and Sohlh2 in mice has been reported in previous studies. Sohlh1 and Sohlh2 are specifically expressed in spermatogonia, prespermatogonia in male mice and oocytes of primordial and primary follicles in female mice. In this report, we studied the expression pattern of Sohlh1 and Sohlh2 in human adult tissues. Immunohistochemical staining of Sohlh1 and Sohlh2 was performed in 5 samples of normal ovaries and testes, respectively. The results revealed that Sohlh genes are not only expressed in oocytes and spermatogonia, but also in granular cells, theca cells, Sertoli cells and Leydig cells, and in smooth muscles of blood vessel walls. To further investigate the expression of Sohlh genes in other adult human tissues, we collected representative normal adult tissues developed from three embryonic germ layers. Compared with the expression in mice, Sohlhs exhibited a much more extensive expression pattern in human tissues. Sohlhs were detected in testis, ovary and epithelia developed from embryonic endoderm, ectoderm and tissues developed from embryonic mesoderm. Sohlh signals were found in spermatogonia, Sertoli cells and also Leydig cells in testis, while in ovary, the expression was mainly in oocytes of primordial and primary follicles, granular cells and theca cells of secondary follicles. Compared with Sohlh2, the expression of Sohlh1 was stronger and more extensive. Our study explored the expression of Sohlh genes in human tissues and might provide insights for functional studies of Sohlh genes.
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spelling pubmed-45742032015-09-18 Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues Zhang, Xiaoli Liu, Ruihua Su, Zhongxue Zhang, Yuecun Zhang, Wenfang Liu, Xinyu Wang, Fuwu Guo, Yuji Li, Chuangang Hao, Jing PLoS One Research Article The expression pattern of Sohlh1 (spermatogenesis and oogenesis specific basic helix-loop-helix 1) and Sohlh2 in mice has been reported in previous studies. Sohlh1 and Sohlh2 are specifically expressed in spermatogonia, prespermatogonia in male mice and oocytes of primordial and primary follicles in female mice. In this report, we studied the expression pattern of Sohlh1 and Sohlh2 in human adult tissues. Immunohistochemical staining of Sohlh1 and Sohlh2 was performed in 5 samples of normal ovaries and testes, respectively. The results revealed that Sohlh genes are not only expressed in oocytes and spermatogonia, but also in granular cells, theca cells, Sertoli cells and Leydig cells, and in smooth muscles of blood vessel walls. To further investigate the expression of Sohlh genes in other adult human tissues, we collected representative normal adult tissues developed from three embryonic germ layers. Compared with the expression in mice, Sohlhs exhibited a much more extensive expression pattern in human tissues. Sohlhs were detected in testis, ovary and epithelia developed from embryonic endoderm, ectoderm and tissues developed from embryonic mesoderm. Sohlh signals were found in spermatogonia, Sertoli cells and also Leydig cells in testis, while in ovary, the expression was mainly in oocytes of primordial and primary follicles, granular cells and theca cells of secondary follicles. Compared with Sohlh2, the expression of Sohlh1 was stronger and more extensive. Our study explored the expression of Sohlh genes in human tissues and might provide insights for functional studies of Sohlh genes. Public Library of Science 2015-09-16 /pmc/articles/PMC4574203/ /pubmed/26375665 http://dx.doi.org/10.1371/journal.pone.0137431 Text en © 2015 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Xiaoli
Liu, Ruihua
Su, Zhongxue
Zhang, Yuecun
Zhang, Wenfang
Liu, Xinyu
Wang, Fuwu
Guo, Yuji
Li, Chuangang
Hao, Jing
Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues
title Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues
title_full Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues
title_fullStr Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues
title_full_unstemmed Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues
title_short Immunohistochemical Study of Expression of Sohlh1 and Sohlh2 in Normal Adult Human Tissues
title_sort immunohistochemical study of expression of sohlh1 and sohlh2 in normal adult human tissues
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574203/
https://www.ncbi.nlm.nih.gov/pubmed/26375665
http://dx.doi.org/10.1371/journal.pone.0137431
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