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QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids
BACKGROUND: Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cloning. Their widespread use, however, is hampered by some of their inherent cha...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574722/ https://www.ncbi.nlm.nih.gov/pubmed/26388935 http://dx.doi.org/10.1186/s13036-015-0010-3 |