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Expedited quantification of mutant ribosomal RNA by binary deoxyribozyme (BiDz) sensors

Mutations in ribosomal RNA (rRNA) have traditionally been detected by the primer extension assay, which is a tedious and multistage procedure. Here, we describe a simple and straightforward fluorescence assay based on binary deoxyribozyme (BiDz) sensors. The assay uses two short DNA oligonucleotides...

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Detalles Bibliográficos
Autores principales:	Gerasimova, Yulia V., Yakovchuk, Petro, Dedkova, Larisa M., Hecht, Sidney M., Kolpashchikov, Dmitry M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2015
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4574759/ https://www.ncbi.nlm.nih.gov/pubmed/26289345 http://dx.doi.org/10.1261/rna.052613.115

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