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microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1

To explore the potential roles of miRNAs in controlling the survival of mycobacteria in macrophages, miR-17-5p in the regulation of Bacillus Calmette-Guérin(BCG)growth in the macrophage RAW264.7 cells was interrogated. Our results reveal that an infection of BCG shows a time-dependent up-regulation...

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Autores principales: Duan, Xiangguo, Zhang, Tao, Ding, Shuqin, Wei, Jun, Su, Chunxia, Liu, Hongpeng, Xu, Guangxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575043/
https://www.ncbi.nlm.nih.gov/pubmed/26384021
http://dx.doi.org/10.1371/journal.pone.0138011
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author Duan, Xiangguo
Zhang, Tao
Ding, Shuqin
Wei, Jun
Su, Chunxia
Liu, Hongpeng
Xu, Guangxian
author_facet Duan, Xiangguo
Zhang, Tao
Ding, Shuqin
Wei, Jun
Su, Chunxia
Liu, Hongpeng
Xu, Guangxian
author_sort Duan, Xiangguo
collection PubMed
description To explore the potential roles of miRNAs in controlling the survival of mycobacteria in macrophages, miR-17-5p in the regulation of Bacillus Calmette-Guérin(BCG)growth in the macrophage RAW264.7 cells was interrogated. Our results reveal that an infection of BCG shows a time-dependent up-regulation of miR-17-5p in RAW264.7 cells in early phase; importantly, excessive expression of miR-17-5p in these cells exhibits an increased propagation of intracellular BCG. Mechanistically, the Unc-51 like autophagy activating kinase 1 (ULK1), an initial molecular of autophagy are identified as novel target of miR-17-5p, the miR-17-5p is capable of targeting down-regulating the expression of ULK1 protein. In addition, an overexpression of miR-17-5p in RAW264.7 cells is correlated with repression of ULK1 and the autophagosome related proteins LC3I/II. These results imply that miR-17-5p may be able to arrest the maturation of mycobacterial phagosomes in part by targeting ULK1, subsequently reduces the ability of host cells to kill intracellular BCG.
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spelling pubmed-45750432015-09-25 microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1 Duan, Xiangguo Zhang, Tao Ding, Shuqin Wei, Jun Su, Chunxia Liu, Hongpeng Xu, Guangxian PLoS One Research Article To explore the potential roles of miRNAs in controlling the survival of mycobacteria in macrophages, miR-17-5p in the regulation of Bacillus Calmette-Guérin(BCG)growth in the macrophage RAW264.7 cells was interrogated. Our results reveal that an infection of BCG shows a time-dependent up-regulation of miR-17-5p in RAW264.7 cells in early phase; importantly, excessive expression of miR-17-5p in these cells exhibits an increased propagation of intracellular BCG. Mechanistically, the Unc-51 like autophagy activating kinase 1 (ULK1), an initial molecular of autophagy are identified as novel target of miR-17-5p, the miR-17-5p is capable of targeting down-regulating the expression of ULK1 protein. In addition, an overexpression of miR-17-5p in RAW264.7 cells is correlated with repression of ULK1 and the autophagosome related proteins LC3I/II. These results imply that miR-17-5p may be able to arrest the maturation of mycobacterial phagosomes in part by targeting ULK1, subsequently reduces the ability of host cells to kill intracellular BCG. Public Library of Science 2015-09-18 /pmc/articles/PMC4575043/ /pubmed/26384021 http://dx.doi.org/10.1371/journal.pone.0138011 Text en © 2015 Duan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Duan, Xiangguo
Zhang, Tao
Ding, Shuqin
Wei, Jun
Su, Chunxia
Liu, Hongpeng
Xu, Guangxian
microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1
title microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1
title_full microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1
title_fullStr microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1
title_full_unstemmed microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1
title_short microRNA-17-5p Modulates Bacille Calmette-Guerin Growth in RAW264.7 Cells by Targeting ULK1
title_sort microrna-17-5p modulates bacille calmette-guerin growth in raw264.7 cells by targeting ulk1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575043/
https://www.ncbi.nlm.nih.gov/pubmed/26384021
http://dx.doi.org/10.1371/journal.pone.0138011
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