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Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance
Mechanisms of plant root tolerance to high temperatures through antioxidant defense are not well understood. The objective of this study was to investigate whether superior root thermotolerance of heat-tolerant Agrostis scabra relative to its congeneric heat-sensitive Agrostis stolonifera was associ...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575078/ https://www.ncbi.nlm.nih.gov/pubmed/26382960 http://dx.doi.org/10.1371/journal.pone.0138268 |
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author | Xu, Yi Burgess, Patrick Huang, Bingru |
author_facet | Xu, Yi Burgess, Patrick Huang, Bingru |
author_sort | Xu, Yi |
collection | PubMed |
description | Mechanisms of plant root tolerance to high temperatures through antioxidant defense are not well understood. The objective of this study was to investigate whether superior root thermotolerance of heat-tolerant Agrostis scabra relative to its congeneric heat-sensitive Agrostis stolonifera was associated with differential accumulation of reactive oxygen species and antioxidant scavenging systems. A. scabra ‘NTAS’ and A. stolonifera ‘Penncross’ plants were exposed to heat stress (35/30°C, day/night) in growth chambers for 24 d. Superoxide (O(2) (-)) content increased in both A. stolonifera and A. scabra roots under heat stress but to a far lesser extent in A. scabra than in A. stolonifera. Hydrogen peroxide (H(2)O(2)) content increased significantly in A. stolonifera roots but not in A. scabra roots responding to heat stress. The content of antioxidant compounds (ascorbate and glutathione) did not differ between A. stolonifera and A. scabra under heat stress. Enzymatic activity of superoxide dismutase was less suppressed in A. scabra than that in A. stolonifera under heat stress, while peroxidase and catalase were more induced in A. scabra than in A. stolonifera. Similarly, their encoded transcript levels were either less suppressed, or more induced in A. scabra roots than those in A. stolonifera during heat stress. Roots of A. scabra exhibited greater alternative respiration rate and lower cytochrome respiration rate under heat stress, which was associated with suppression of O(2) (-) and H(2)O(2) production as shown by respiration inhibitors. Superior root thermotolerance of A. scabra was related to decreases in H(2)O(2) and O(2) (-) accumulation facilitated by active enzymatic antioxidant defense systems and the maintenance of alternative respiration, alleviating cellular damages by heat-induced oxidative stress. |
format | Online Article Text |
id | pubmed-4575078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45750782015-09-25 Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance Xu, Yi Burgess, Patrick Huang, Bingru PLoS One Research Article Mechanisms of plant root tolerance to high temperatures through antioxidant defense are not well understood. The objective of this study was to investigate whether superior root thermotolerance of heat-tolerant Agrostis scabra relative to its congeneric heat-sensitive Agrostis stolonifera was associated with differential accumulation of reactive oxygen species and antioxidant scavenging systems. A. scabra ‘NTAS’ and A. stolonifera ‘Penncross’ plants were exposed to heat stress (35/30°C, day/night) in growth chambers for 24 d. Superoxide (O(2) (-)) content increased in both A. stolonifera and A. scabra roots under heat stress but to a far lesser extent in A. scabra than in A. stolonifera. Hydrogen peroxide (H(2)O(2)) content increased significantly in A. stolonifera roots but not in A. scabra roots responding to heat stress. The content of antioxidant compounds (ascorbate and glutathione) did not differ between A. stolonifera and A. scabra under heat stress. Enzymatic activity of superoxide dismutase was less suppressed in A. scabra than that in A. stolonifera under heat stress, while peroxidase and catalase were more induced in A. scabra than in A. stolonifera. Similarly, their encoded transcript levels were either less suppressed, or more induced in A. scabra roots than those in A. stolonifera during heat stress. Roots of A. scabra exhibited greater alternative respiration rate and lower cytochrome respiration rate under heat stress, which was associated with suppression of O(2) (-) and H(2)O(2) production as shown by respiration inhibitors. Superior root thermotolerance of A. scabra was related to decreases in H(2)O(2) and O(2) (-) accumulation facilitated by active enzymatic antioxidant defense systems and the maintenance of alternative respiration, alleviating cellular damages by heat-induced oxidative stress. Public Library of Science 2015-09-18 /pmc/articles/PMC4575078/ /pubmed/26382960 http://dx.doi.org/10.1371/journal.pone.0138268 Text en © 2015 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xu, Yi Burgess, Patrick Huang, Bingru Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance |
title | Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance |
title_full | Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance |
title_fullStr | Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance |
title_full_unstemmed | Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance |
title_short | Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance |
title_sort | root antioxidant mechanisms in relation to root thermotolerance in perennial grass species contrasting in heat tolerance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575078/ https://www.ncbi.nlm.nih.gov/pubmed/26382960 http://dx.doi.org/10.1371/journal.pone.0138268 |
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