Cargando…

Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance

Mechanisms of plant root tolerance to high temperatures through antioxidant defense are not well understood. The objective of this study was to investigate whether superior root thermotolerance of heat-tolerant Agrostis scabra relative to its congeneric heat-sensitive Agrostis stolonifera was associ...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Yi, Burgess, Patrick, Huang, Bingru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575078/
https://www.ncbi.nlm.nih.gov/pubmed/26382960
http://dx.doi.org/10.1371/journal.pone.0138268
_version_ 1782390724246896640
author Xu, Yi
Burgess, Patrick
Huang, Bingru
author_facet Xu, Yi
Burgess, Patrick
Huang, Bingru
author_sort Xu, Yi
collection PubMed
description Mechanisms of plant root tolerance to high temperatures through antioxidant defense are not well understood. The objective of this study was to investigate whether superior root thermotolerance of heat-tolerant Agrostis scabra relative to its congeneric heat-sensitive Agrostis stolonifera was associated with differential accumulation of reactive oxygen species and antioxidant scavenging systems. A. scabra ‘NTAS’ and A. stolonifera ‘Penncross’ plants were exposed to heat stress (35/30°C, day/night) in growth chambers for 24 d. Superoxide (O(2) (-)) content increased in both A. stolonifera and A. scabra roots under heat stress but to a far lesser extent in A. scabra than in A. stolonifera. Hydrogen peroxide (H(2)O(2)) content increased significantly in A. stolonifera roots but not in A. scabra roots responding to heat stress. The content of antioxidant compounds (ascorbate and glutathione) did not differ between A. stolonifera and A. scabra under heat stress. Enzymatic activity of superoxide dismutase was less suppressed in A. scabra than that in A. stolonifera under heat stress, while peroxidase and catalase were more induced in A. scabra than in A. stolonifera. Similarly, their encoded transcript levels were either less suppressed, or more induced in A. scabra roots than those in A. stolonifera during heat stress. Roots of A. scabra exhibited greater alternative respiration rate and lower cytochrome respiration rate under heat stress, which was associated with suppression of O(2) (-) and H(2)O(2) production as shown by respiration inhibitors. Superior root thermotolerance of A. scabra was related to decreases in H(2)O(2) and O(2) (-) accumulation facilitated by active enzymatic antioxidant defense systems and the maintenance of alternative respiration, alleviating cellular damages by heat-induced oxidative stress.
format Online
Article
Text
id pubmed-4575078
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45750782015-09-25 Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance Xu, Yi Burgess, Patrick Huang, Bingru PLoS One Research Article Mechanisms of plant root tolerance to high temperatures through antioxidant defense are not well understood. The objective of this study was to investigate whether superior root thermotolerance of heat-tolerant Agrostis scabra relative to its congeneric heat-sensitive Agrostis stolonifera was associated with differential accumulation of reactive oxygen species and antioxidant scavenging systems. A. scabra ‘NTAS’ and A. stolonifera ‘Penncross’ plants were exposed to heat stress (35/30°C, day/night) in growth chambers for 24 d. Superoxide (O(2) (-)) content increased in both A. stolonifera and A. scabra roots under heat stress but to a far lesser extent in A. scabra than in A. stolonifera. Hydrogen peroxide (H(2)O(2)) content increased significantly in A. stolonifera roots but not in A. scabra roots responding to heat stress. The content of antioxidant compounds (ascorbate and glutathione) did not differ between A. stolonifera and A. scabra under heat stress. Enzymatic activity of superoxide dismutase was less suppressed in A. scabra than that in A. stolonifera under heat stress, while peroxidase and catalase were more induced in A. scabra than in A. stolonifera. Similarly, their encoded transcript levels were either less suppressed, or more induced in A. scabra roots than those in A. stolonifera during heat stress. Roots of A. scabra exhibited greater alternative respiration rate and lower cytochrome respiration rate under heat stress, which was associated with suppression of O(2) (-) and H(2)O(2) production as shown by respiration inhibitors. Superior root thermotolerance of A. scabra was related to decreases in H(2)O(2) and O(2) (-) accumulation facilitated by active enzymatic antioxidant defense systems and the maintenance of alternative respiration, alleviating cellular damages by heat-induced oxidative stress. Public Library of Science 2015-09-18 /pmc/articles/PMC4575078/ /pubmed/26382960 http://dx.doi.org/10.1371/journal.pone.0138268 Text en © 2015 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xu, Yi
Burgess, Patrick
Huang, Bingru
Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance
title Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance
title_full Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance
title_fullStr Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance
title_full_unstemmed Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance
title_short Root Antioxidant Mechanisms in Relation to Root Thermotolerance in Perennial Grass Species Contrasting in Heat Tolerance
title_sort root antioxidant mechanisms in relation to root thermotolerance in perennial grass species contrasting in heat tolerance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575078/
https://www.ncbi.nlm.nih.gov/pubmed/26382960
http://dx.doi.org/10.1371/journal.pone.0138268
work_keys_str_mv AT xuyi rootantioxidantmechanismsinrelationtorootthermotoleranceinperennialgrassspeciescontrastinginheattolerance
AT burgesspatrick rootantioxidantmechanismsinrelationtorootthermotoleranceinperennialgrassspeciescontrastinginheattolerance
AT huangbingru rootantioxidantmechanismsinrelationtorootthermotoleranceinperennialgrassspeciescontrastinginheattolerance