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Accelerated clearance of human red blood cells in a rat transfusion model

BACKGROUND: Animal models are valuable in transfusion research. Use of human red blood cells (RBCs) in animal models facilitates extrapolation of the impact of storage conditions to the human condition but may be hampered by the use of cross species. METHODS: Investigation of clearance and posttrans...

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Autores principales: Straat, M., Klei, TRL, de Korte, D., van Bruggen, R., Juffermans, NP
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575311/
https://www.ncbi.nlm.nih.gov/pubmed/26384491
http://dx.doi.org/10.1186/s40635-015-0064-z
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author Straat, M.
Klei, TRL
de Korte, D.
van Bruggen, R.
Juffermans, NP
author_facet Straat, M.
Klei, TRL
de Korte, D.
van Bruggen, R.
Juffermans, NP
author_sort Straat, M.
collection PubMed
description BACKGROUND: Animal models are valuable in transfusion research. Use of human red blood cells (RBCs) in animal models facilitates extrapolation of the impact of storage conditions to the human condition but may be hampered by the use of cross species. METHODS: Investigation of clearance and posttransfusion recovery in a rat model using fresh and stored human RBCs. RESULTS: Directly following transfusion, human RBCs could be detected in the circulation of all recipients, with higher recovery rates for stored RBCs than for fresh RBCs. After 24 h following transfusion, no donor RBCs could be detected in the circulation, but donor RBCs could be detected in all organs of all recipients. CONCLUSION: The use of human donor RBCs in a rat transfusion model resulted in clearance from cells from the circulation. Donor cells were found in different organs of the recipients. Rat transfusion models are thus not appropriate to study the efficacy of human RBC transfusion.
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spelling pubmed-45753112015-09-23 Accelerated clearance of human red blood cells in a rat transfusion model Straat, M. Klei, TRL de Korte, D. van Bruggen, R. Juffermans, NP Intensive Care Med Exp Methodology BACKGROUND: Animal models are valuable in transfusion research. Use of human red blood cells (RBCs) in animal models facilitates extrapolation of the impact of storage conditions to the human condition but may be hampered by the use of cross species. METHODS: Investigation of clearance and posttransfusion recovery in a rat model using fresh and stored human RBCs. RESULTS: Directly following transfusion, human RBCs could be detected in the circulation of all recipients, with higher recovery rates for stored RBCs than for fresh RBCs. After 24 h following transfusion, no donor RBCs could be detected in the circulation, but donor RBCs could be detected in all organs of all recipients. CONCLUSION: The use of human donor RBCs in a rat transfusion model resulted in clearance from cells from the circulation. Donor cells were found in different organs of the recipients. Rat transfusion models are thus not appropriate to study the efficacy of human RBC transfusion. Springer International Publishing 2015-09-18 /pmc/articles/PMC4575311/ /pubmed/26384491 http://dx.doi.org/10.1186/s40635-015-0064-z Text en © Straat et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Methodology
Straat, M.
Klei, TRL
de Korte, D.
van Bruggen, R.
Juffermans, NP
Accelerated clearance of human red blood cells in a rat transfusion model
title Accelerated clearance of human red blood cells in a rat transfusion model
title_full Accelerated clearance of human red blood cells in a rat transfusion model
title_fullStr Accelerated clearance of human red blood cells in a rat transfusion model
title_full_unstemmed Accelerated clearance of human red blood cells in a rat transfusion model
title_short Accelerated clearance of human red blood cells in a rat transfusion model
title_sort accelerated clearance of human red blood cells in a rat transfusion model
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575311/
https://www.ncbi.nlm.nih.gov/pubmed/26384491
http://dx.doi.org/10.1186/s40635-015-0064-z
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