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The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation

BACKGROUND: Expression of kisspeptin (protein) and Kiss1r (mRNA) was recently documented in the mouse uterus on D4 of pregnancy (the day of embryo implantation) suggesting that the uterine-based kisspeptin (KP)/kisspeptin receptor (KISS1R) signaling system regulates embryo implantation. Despite this...

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Autores principales: Fayazi, Mehri, Calder, Michele, Bhattacharya, Moshmi, Vilos, George A., Power, Stephen, Babwah, Andy V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575475/
https://www.ncbi.nlm.nih.gov/pubmed/26384646
http://dx.doi.org/10.1186/s12958-015-0105-1
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author Fayazi, Mehri
Calder, Michele
Bhattacharya, Moshmi
Vilos, George A.
Power, Stephen
Babwah, Andy V.
author_facet Fayazi, Mehri
Calder, Michele
Bhattacharya, Moshmi
Vilos, George A.
Power, Stephen
Babwah, Andy V.
author_sort Fayazi, Mehri
collection PubMed
description BACKGROUND: Expression of kisspeptin (protein) and Kiss1r (mRNA) was recently documented in the mouse uterus on D4 of pregnancy (the day of embryo implantation) suggesting that the uterine-based kisspeptin (KP)/kisspeptin receptor (KISS1R) signaling system regulates embryo implantation. Despite this important suggestion, it was never demonstrated that the uterus actually exhibits a functional KP/KISS1R signaling system on D4 of pregnancy. Thus, the goal of this study was to determine whether a functional KP/KISS1R signaling system exists in the mouse uterus on D4 of pregnancy. FINDINGS: Since kisspeptin/KISS1R signaling triggers the phosphorylation of the mitogen-activated protein kinases p38 and ERK1/2, through immunohistochemical analyses, we determined whether exogenously administered kisspeptin could trigger p38 and ERK1/2 phosphorylation in the uterus on D4 of pregnancy. The results clearly demonstrated that kisspeptin could and that its effects were mediated via KISS1R. Additionally, the robust kisspeptin-triggered response was observed in the pregnant uterus only. Finally, it was demonstrated that on D4 of pregnancy the Kiss1 null uterus expresses functional KISS1R molecules capable of mediating the effects of kisspeptin. CONCLUSIONS: These results lead us to conclude that on D4 of pregnancy, the mouse uterus expresses a functional KP/KISS1R signaling system strengthening the possibility that this signaling system regulates embryo implantation. These findings strengthen the rationale for determining whether such a functional system exists in the uterus of the human female and if so, what role it might play in human pregnancy.
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spelling pubmed-45754752015-09-20 The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation Fayazi, Mehri Calder, Michele Bhattacharya, Moshmi Vilos, George A. Power, Stephen Babwah, Andy V. Reprod Biol Endocrinol Short Communication BACKGROUND: Expression of kisspeptin (protein) and Kiss1r (mRNA) was recently documented in the mouse uterus on D4 of pregnancy (the day of embryo implantation) suggesting that the uterine-based kisspeptin (KP)/kisspeptin receptor (KISS1R) signaling system regulates embryo implantation. Despite this important suggestion, it was never demonstrated that the uterus actually exhibits a functional KP/KISS1R signaling system on D4 of pregnancy. Thus, the goal of this study was to determine whether a functional KP/KISS1R signaling system exists in the mouse uterus on D4 of pregnancy. FINDINGS: Since kisspeptin/KISS1R signaling triggers the phosphorylation of the mitogen-activated protein kinases p38 and ERK1/2, through immunohistochemical analyses, we determined whether exogenously administered kisspeptin could trigger p38 and ERK1/2 phosphorylation in the uterus on D4 of pregnancy. The results clearly demonstrated that kisspeptin could and that its effects were mediated via KISS1R. Additionally, the robust kisspeptin-triggered response was observed in the pregnant uterus only. Finally, it was demonstrated that on D4 of pregnancy the Kiss1 null uterus expresses functional KISS1R molecules capable of mediating the effects of kisspeptin. CONCLUSIONS: These results lead us to conclude that on D4 of pregnancy, the mouse uterus expresses a functional KP/KISS1R signaling system strengthening the possibility that this signaling system regulates embryo implantation. These findings strengthen the rationale for determining whether such a functional system exists in the uterus of the human female and if so, what role it might play in human pregnancy. BioMed Central 2015-09-18 /pmc/articles/PMC4575475/ /pubmed/26384646 http://dx.doi.org/10.1186/s12958-015-0105-1 Text en © Fayazi et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Communication
Fayazi, Mehri
Calder, Michele
Bhattacharya, Moshmi
Vilos, George A.
Power, Stephen
Babwah, Andy V.
The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation
title The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation
title_full The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation
title_fullStr The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation
title_full_unstemmed The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation
title_short The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation
title_sort pregnant mouse uterus exhibits a functional kisspeptin/kiss1r signaling system on the day of embryo implantation
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575475/
https://www.ncbi.nlm.nih.gov/pubmed/26384646
http://dx.doi.org/10.1186/s12958-015-0105-1
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