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Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats

BACKGROUND: Recent studies have shown that benzene extract of Ocimum sanctum (O. sanctum) leaves induces the ultrastructural changes in the epithelial cells of the cauda epididymis, its subsequent recovery in the seminiferous epithelium and fertility of male albino rats. OBJECTIVE: Our aim was to in...

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Autores principales: Ahmed, Mukhtar, Ahamed, R. Nazeer, H Aladakatti, Ravindranath, G Ghodesawar, Mukhtar Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Clinical Center for Infertility 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575751/
https://www.ncbi.nlm.nih.gov/pubmed/26396561
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author Ahmed, Mukhtar
Ahamed, R. Nazeer
H Aladakatti, Ravindranath
G Ghodesawar, Mukhtar Ahmed
author_facet Ahmed, Mukhtar
Ahamed, R. Nazeer
H Aladakatti, Ravindranath
G Ghodesawar, Mukhtar Ahmed
author_sort Ahmed, Mukhtar
collection PubMed
description BACKGROUND: Recent studies have shown that benzene extract of Ocimum sanctum (O. sanctum) leaves induces the ultrastructural changes in the epithelial cells of the cauda epididymis, its subsequent recovery in the seminiferous epithelium and fertility of male albino rats. OBJECTIVE: Our aim was to investigate the effect of benzene extract of O.sanctum leaves on the cauda epididymal sperm parameters, morphology and their organelles at the ultrastructural level in albino rats. MATERIALS AND METHODS: Wistar male rats (n=20) were allocated into two groups of control (n=10) and test group (n=10). The test group received benzene extract of O.sanctum leaves (250mg/kg/day) for 48 consequence days. Five animals from each group were used for fertility test. Twenty-four hours after the last dose, the rest of the control (n=5) and treated (n=5) animals were sacrificed by cervical dislocation and then the cauda epididymal plasma was used for sperm analysis, scanning electron microscopy (SEM) and transmission electron microscopic (TEM) studies. RESULTS: Sperm analysis of test group exhibited significant (p≤0.001) decrease in the sperm count, motility, speed and increase in sperm anomalies when compare to control group. SEM and TEM observation in treated animals indicated the morphological changes in plasma membrane as well as in the acrosomal membrane of spermatozoa, formation of a balloon-like cytoplasmic droplet in the mid-region of abnormal tail and disorganization or degeneration of mitochondria of sperm mitochondrial sheaths. CONCLUSION: The effects observed in this study may have resulted from a general alteration in the cauda epididymal milieu, probably due to androgen deficiency consequent to the anti-androgenic property of O.sanctum leaves.
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spelling pubmed-45757512015-09-22 Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats Ahmed, Mukhtar Ahamed, R. Nazeer H Aladakatti, Ravindranath G Ghodesawar, Mukhtar Ahmed Iran J Reprod Med Original Article BACKGROUND: Recent studies have shown that benzene extract of Ocimum sanctum (O. sanctum) leaves induces the ultrastructural changes in the epithelial cells of the cauda epididymis, its subsequent recovery in the seminiferous epithelium and fertility of male albino rats. OBJECTIVE: Our aim was to investigate the effect of benzene extract of O.sanctum leaves on the cauda epididymal sperm parameters, morphology and their organelles at the ultrastructural level in albino rats. MATERIALS AND METHODS: Wistar male rats (n=20) were allocated into two groups of control (n=10) and test group (n=10). The test group received benzene extract of O.sanctum leaves (250mg/kg/day) for 48 consequence days. Five animals from each group were used for fertility test. Twenty-four hours after the last dose, the rest of the control (n=5) and treated (n=5) animals were sacrificed by cervical dislocation and then the cauda epididymal plasma was used for sperm analysis, scanning electron microscopy (SEM) and transmission electron microscopic (TEM) studies. RESULTS: Sperm analysis of test group exhibited significant (p≤0.001) decrease in the sperm count, motility, speed and increase in sperm anomalies when compare to control group. SEM and TEM observation in treated animals indicated the morphological changes in plasma membrane as well as in the acrosomal membrane of spermatozoa, formation of a balloon-like cytoplasmic droplet in the mid-region of abnormal tail and disorganization or degeneration of mitochondria of sperm mitochondrial sheaths. CONCLUSION: The effects observed in this study may have resulted from a general alteration in the cauda epididymal milieu, probably due to androgen deficiency consequent to the anti-androgenic property of O.sanctum leaves. Research and Clinical Center for Infertility 2011 /pmc/articles/PMC4575751/ /pubmed/26396561 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ahmed, Mukhtar
Ahamed, R. Nazeer
H Aladakatti, Ravindranath
G Ghodesawar, Mukhtar Ahmed
Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats
title Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats
title_full Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats
title_fullStr Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats
title_full_unstemmed Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats
title_short Effect of benzene extract of Ocimum sanctum leaves on cauda epididymal spermatozoa of rats
title_sort effect of benzene extract of ocimum sanctum leaves on cauda epididymal spermatozoa of rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575751/
https://www.ncbi.nlm.nih.gov/pubmed/26396561
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