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Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens

Exosomes are nano-sized vesicles released by cells into the extracellular space and have been shown to be present in thymic tissue both in mice and in humans. The source of thymic exosomes is however still an enigma and hence it is not known whether thymic epithelial cells (TECs) are able to produce...

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Autores principales: Skogberg, Gabriel, Lundberg, Vanja, Berglund, Martin, Gudmundsdottir, Judith, Telemo, Esbjörn, Lindgren, Susanne, Ekwall, Olov
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575951/
https://www.ncbi.nlm.nih.gov/pubmed/25776846
http://dx.doi.org/10.1038/icb.2015.33
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author Skogberg, Gabriel
Lundberg, Vanja
Berglund, Martin
Gudmundsdottir, Judith
Telemo, Esbjörn
Lindgren, Susanne
Ekwall, Olov
author_facet Skogberg, Gabriel
Lundberg, Vanja
Berglund, Martin
Gudmundsdottir, Judith
Telemo, Esbjörn
Lindgren, Susanne
Ekwall, Olov
author_sort Skogberg, Gabriel
collection PubMed
description Exosomes are nano-sized vesicles released by cells into the extracellular space and have been shown to be present in thymic tissue both in mice and in humans. The source of thymic exosomes is however still an enigma and hence it is not known whether thymic epithelial cells (TECs) are able to produce exosomes. In this work, we have cultured human TECs and isolated exosomes. These exosomes carry tissue-restricted antigens (TRAs), for example, myelin basic protein and desmoglein 3. The presence of TRAs indicates a possible role for thymic epithelium-derived exosomes in the selection process of thymocytes. The key contribution of these exosomes could be to disseminate self-antigens from the thymic epithelia, thus making them more accessible to the pool of maturing thymocytes. This would increase the coverage of TRAs within the thymus, and facilitate the process of positive and negative selection.
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spelling pubmed-45759512015-09-30 Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens Skogberg, Gabriel Lundberg, Vanja Berglund, Martin Gudmundsdottir, Judith Telemo, Esbjörn Lindgren, Susanne Ekwall, Olov Immunol Cell Biol Original Article Exosomes are nano-sized vesicles released by cells into the extracellular space and have been shown to be present in thymic tissue both in mice and in humans. The source of thymic exosomes is however still an enigma and hence it is not known whether thymic epithelial cells (TECs) are able to produce exosomes. In this work, we have cultured human TECs and isolated exosomes. These exosomes carry tissue-restricted antigens (TRAs), for example, myelin basic protein and desmoglein 3. The presence of TRAs indicates a possible role for thymic epithelium-derived exosomes in the selection process of thymocytes. The key contribution of these exosomes could be to disseminate self-antigens from the thymic epithelia, thus making them more accessible to the pool of maturing thymocytes. This would increase the coverage of TRAs within the thymus, and facilitate the process of positive and negative selection. Nature Publishing Group 2015-09 2015-03-17 /pmc/articles/PMC4575951/ /pubmed/25776846 http://dx.doi.org/10.1038/icb.2015.33 Text en Copyright © 2015 Australasian Society for Immunology Inc. http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Original Article
Skogberg, Gabriel
Lundberg, Vanja
Berglund, Martin
Gudmundsdottir, Judith
Telemo, Esbjörn
Lindgren, Susanne
Ekwall, Olov
Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens
title Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens
title_full Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens
title_fullStr Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens
title_full_unstemmed Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens
title_short Human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens
title_sort human thymic epithelial primary cells produce exosomes carrying tissue-restricted antigens
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575951/
https://www.ncbi.nlm.nih.gov/pubmed/25776846
http://dx.doi.org/10.1038/icb.2015.33
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