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In vivo assay to monitor flavonoid uptake across plant cell membranes
Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575953/ https://www.ncbi.nlm.nih.gov/pubmed/26504740 http://dx.doi.org/10.1016/j.fob.2015.08.009 |
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author | Filippi, Antonio Petrussa, Elisa Peresson, Carlo Bertolini, Alberto Vianello, Angelo Braidot, Enrico |
author_facet | Filippi, Antonio Petrussa, Elisa Peresson, Carlo Bertolini, Alberto Vianello, Angelo Braidot, Enrico |
author_sort | Filippi, Antonio |
collection | PubMed |
description | Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant tissues remains unclear. This is due to the absence of a convenient and simple method to monitor their transport. In the present work, we suggest an assay able to follow in vivo transport of quercetin, the most abundant flavonoid in plant tissues. This uptake was monitored using 2-aminoethoxydiphenyl borate (DPBA), a fluorescent probe, in non-pigmented Vitis vinifera cell cultures. |
format | Online Article Text |
id | pubmed-4575953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-45759532015-10-26 In vivo assay to monitor flavonoid uptake across plant cell membranes Filippi, Antonio Petrussa, Elisa Peresson, Carlo Bertolini, Alberto Vianello, Angelo Braidot, Enrico FEBS Open Bio Method Flavonoids represent one of the most important molecules of plant secondary metabolism, playing many different biochemical and physiological roles. Although their essential role in plant life and human health has been elucidated by many studies, their subcellular transport and accumulation in plant tissues remains unclear. This is due to the absence of a convenient and simple method to monitor their transport. In the present work, we suggest an assay able to follow in vivo transport of quercetin, the most abundant flavonoid in plant tissues. This uptake was monitored using 2-aminoethoxydiphenyl borate (DPBA), a fluorescent probe, in non-pigmented Vitis vinifera cell cultures. Elsevier 2015-08-29 /pmc/articles/PMC4575953/ /pubmed/26504740 http://dx.doi.org/10.1016/j.fob.2015.08.009 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Method Filippi, Antonio Petrussa, Elisa Peresson, Carlo Bertolini, Alberto Vianello, Angelo Braidot, Enrico In vivo assay to monitor flavonoid uptake across plant cell membranes |
title | In vivo assay to monitor flavonoid uptake across plant cell membranes |
title_full | In vivo assay to monitor flavonoid uptake across plant cell membranes |
title_fullStr | In vivo assay to monitor flavonoid uptake across plant cell membranes |
title_full_unstemmed | In vivo assay to monitor flavonoid uptake across plant cell membranes |
title_short | In vivo assay to monitor flavonoid uptake across plant cell membranes |
title_sort | in vivo assay to monitor flavonoid uptake across plant cell membranes |
topic | Method |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575953/ https://www.ncbi.nlm.nih.gov/pubmed/26504740 http://dx.doi.org/10.1016/j.fob.2015.08.009 |
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