Cargando…
Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells
BACKGROUND/OBJECTIVES: We examined the chemical composition and the effect of fermented deer antler on hematopoietic factors in bone marrow cells. MATERIALS/METHODS: For the preparation of fermented deer antler extract (FAB), fermentation was carried out using Bacillus subtilis at 30℃ for 7 days. Th...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Nutrition Society and the Korean Society of Community Nutrition
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575956/ https://www.ncbi.nlm.nih.gov/pubmed/26425273 http://dx.doi.org/10.4162/nrp.2015.9.5.451 |
_version_ | 1782390833118445568 |
---|---|
author | Park, Yooheon Choi, Hyeon-Son Lee, Hyun-Sun Suh, Hyung Joo |
author_facet | Park, Yooheon Choi, Hyeon-Son Lee, Hyun-Sun Suh, Hyung Joo |
author_sort | Park, Yooheon |
collection | PubMed |
description | BACKGROUND/OBJECTIVES: We examined the chemical composition and the effect of fermented deer antler on hematopoietic factors in bone marrow cells. MATERIALS/METHODS: For the preparation of fermented deer antler extract (FAB), fermentation was carried out using Bacillus subtilis at 30℃ for 7 days. The hematopoietic effect of FAB was investigated hematopoietic factors in marrow cells. RESULTS: The contents of total sugar, sulfated glycosaminoglycans, and uronic acid and the dry weight gradually increased with fermentation time. The sialic acid content (from 0.14 mg/mL to 0.54 mg/mL) was the highest on the 4th day of fermentation after which it decreased. The proliferating activity of bone marrow cells increased with fermentation times. The levels of various hematopoietic growth factors were determined to verify the beneficial effect of deer antler extract fermented by B. subtilis on hematopoiesis. FAB increased the number of stem cell factors and granulocyte colony-stimulating factor in bone marrow cells. In addition, FAB augmented the burst-forming unit erythroid and total colonies in splenocyte-conditioned medium compared with non-fermented antler extract (NFA). However, FAB did not affect the mRNA levels of erythropoietin, an important factor for erythropoiesis. CONCLUSIONS: FAB, like NFA, did not directly affect hematopoiesis, but contributed to hematopoiesis by stimulating the production of hematopoietic factors. |
format | Online Article Text |
id | pubmed-4575956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Korean Nutrition Society and the Korean Society of Community Nutrition |
record_format | MEDLINE/PubMed |
spelling | pubmed-45759562015-10-01 Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells Park, Yooheon Choi, Hyeon-Son Lee, Hyun-Sun Suh, Hyung Joo Nutr Res Pract Original Research BACKGROUND/OBJECTIVES: We examined the chemical composition and the effect of fermented deer antler on hematopoietic factors in bone marrow cells. MATERIALS/METHODS: For the preparation of fermented deer antler extract (FAB), fermentation was carried out using Bacillus subtilis at 30℃ for 7 days. The hematopoietic effect of FAB was investigated hematopoietic factors in marrow cells. RESULTS: The contents of total sugar, sulfated glycosaminoglycans, and uronic acid and the dry weight gradually increased with fermentation time. The sialic acid content (from 0.14 mg/mL to 0.54 mg/mL) was the highest on the 4th day of fermentation after which it decreased. The proliferating activity of bone marrow cells increased with fermentation times. The levels of various hematopoietic growth factors were determined to verify the beneficial effect of deer antler extract fermented by B. subtilis on hematopoiesis. FAB increased the number of stem cell factors and granulocyte colony-stimulating factor in bone marrow cells. In addition, FAB augmented the burst-forming unit erythroid and total colonies in splenocyte-conditioned medium compared with non-fermented antler extract (NFA). However, FAB did not affect the mRNA levels of erythropoietin, an important factor for erythropoiesis. CONCLUSIONS: FAB, like NFA, did not directly affect hematopoiesis, but contributed to hematopoiesis by stimulating the production of hematopoietic factors. The Korean Nutrition Society and the Korean Society of Community Nutrition 2015-10 2015-09-10 /pmc/articles/PMC4575956/ /pubmed/26425273 http://dx.doi.org/10.4162/nrp.2015.9.5.451 Text en ©2015 The Korean Nutrition Society and the Korean Society of Community Nutrition http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Park, Yooheon Choi, Hyeon-Son Lee, Hyun-Sun Suh, Hyung Joo Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells |
title | Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells |
title_full | Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells |
title_fullStr | Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells |
title_full_unstemmed | Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells |
title_short | Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells |
title_sort | hematopoietic effect of deer antler extract fermented by bacillus subtilis on murine marrow cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575956/ https://www.ncbi.nlm.nih.gov/pubmed/26425273 http://dx.doi.org/10.4162/nrp.2015.9.5.451 |
work_keys_str_mv | AT parkyooheon hematopoieticeffectofdeerantlerextractfermentedbybacillussubtilisonmurinemarrowcells AT choihyeonson hematopoieticeffectofdeerantlerextractfermentedbybacillussubtilisonmurinemarrowcells AT leehyunsun hematopoieticeffectofdeerantlerextractfermentedbybacillussubtilisonmurinemarrowcells AT suhhyungjoo hematopoieticeffectofdeerantlerextractfermentedbybacillussubtilisonmurinemarrowcells |