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Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells

BACKGROUND/OBJECTIVES: We examined the chemical composition and the effect of fermented deer antler on hematopoietic factors in bone marrow cells. MATERIALS/METHODS: For the preparation of fermented deer antler extract (FAB), fermentation was carried out using Bacillus subtilis at 30℃ for 7 days. Th...

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Autores principales: Park, Yooheon, Choi, Hyeon-Son, Lee, Hyun-Sun, Suh, Hyung Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Nutrition Society and the Korean Society of Community Nutrition 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575956/
https://www.ncbi.nlm.nih.gov/pubmed/26425273
http://dx.doi.org/10.4162/nrp.2015.9.5.451
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author Park, Yooheon
Choi, Hyeon-Son
Lee, Hyun-Sun
Suh, Hyung Joo
author_facet Park, Yooheon
Choi, Hyeon-Son
Lee, Hyun-Sun
Suh, Hyung Joo
author_sort Park, Yooheon
collection PubMed
description BACKGROUND/OBJECTIVES: We examined the chemical composition and the effect of fermented deer antler on hematopoietic factors in bone marrow cells. MATERIALS/METHODS: For the preparation of fermented deer antler extract (FAB), fermentation was carried out using Bacillus subtilis at 30℃ for 7 days. The hematopoietic effect of FAB was investigated hematopoietic factors in marrow cells. RESULTS: The contents of total sugar, sulfated glycosaminoglycans, and uronic acid and the dry weight gradually increased with fermentation time. The sialic acid content (from 0.14 mg/mL to 0.54 mg/mL) was the highest on the 4th day of fermentation after which it decreased. The proliferating activity of bone marrow cells increased with fermentation times. The levels of various hematopoietic growth factors were determined to verify the beneficial effect of deer antler extract fermented by B. subtilis on hematopoiesis. FAB increased the number of stem cell factors and granulocyte colony-stimulating factor in bone marrow cells. In addition, FAB augmented the burst-forming unit erythroid and total colonies in splenocyte-conditioned medium compared with non-fermented antler extract (NFA). However, FAB did not affect the mRNA levels of erythropoietin, an important factor for erythropoiesis. CONCLUSIONS: FAB, like NFA, did not directly affect hematopoiesis, but contributed to hematopoiesis by stimulating the production of hematopoietic factors.
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spelling pubmed-45759562015-10-01 Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells Park, Yooheon Choi, Hyeon-Son Lee, Hyun-Sun Suh, Hyung Joo Nutr Res Pract Original Research BACKGROUND/OBJECTIVES: We examined the chemical composition and the effect of fermented deer antler on hematopoietic factors in bone marrow cells. MATERIALS/METHODS: For the preparation of fermented deer antler extract (FAB), fermentation was carried out using Bacillus subtilis at 30℃ for 7 days. The hematopoietic effect of FAB was investigated hematopoietic factors in marrow cells. RESULTS: The contents of total sugar, sulfated glycosaminoglycans, and uronic acid and the dry weight gradually increased with fermentation time. The sialic acid content (from 0.14 mg/mL to 0.54 mg/mL) was the highest on the 4th day of fermentation after which it decreased. The proliferating activity of bone marrow cells increased with fermentation times. The levels of various hematopoietic growth factors were determined to verify the beneficial effect of deer antler extract fermented by B. subtilis on hematopoiesis. FAB increased the number of stem cell factors and granulocyte colony-stimulating factor in bone marrow cells. In addition, FAB augmented the burst-forming unit erythroid and total colonies in splenocyte-conditioned medium compared with non-fermented antler extract (NFA). However, FAB did not affect the mRNA levels of erythropoietin, an important factor for erythropoiesis. CONCLUSIONS: FAB, like NFA, did not directly affect hematopoiesis, but contributed to hematopoiesis by stimulating the production of hematopoietic factors. The Korean Nutrition Society and the Korean Society of Community Nutrition 2015-10 2015-09-10 /pmc/articles/PMC4575956/ /pubmed/26425273 http://dx.doi.org/10.4162/nrp.2015.9.5.451 Text en ©2015 The Korean Nutrition Society and the Korean Society of Community Nutrition http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Park, Yooheon
Choi, Hyeon-Son
Lee, Hyun-Sun
Suh, Hyung Joo
Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells
title Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells
title_full Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells
title_fullStr Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells
title_full_unstemmed Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells
title_short Hematopoietic effect of deer antler extract fermented by Bacillus subtilis on murine marrow cells
title_sort hematopoietic effect of deer antler extract fermented by bacillus subtilis on murine marrow cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575956/
https://www.ncbi.nlm.nih.gov/pubmed/26425273
http://dx.doi.org/10.4162/nrp.2015.9.5.451
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