In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries

Differential cell systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro selection method for obtaining molecular recognition elements (MREs) that specifically bind to individual cell types with high affinity. MREs are selected from initial large libraries of different nucl...

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Detalles Bibliográficos
Autores principales: Williams, Ryan M., Sooter, Letha J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576012/
https://www.ncbi.nlm.nih.gov/pubmed/26436100
http://dx.doi.org/10.1155/2015/186586
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author Williams, Ryan M.
Sooter, Letha J.
author_facet Williams, Ryan M.
Sooter, Letha J.
author_sort Williams, Ryan M.
collection PubMed
description Differential cell systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro selection method for obtaining molecular recognition elements (MREs) that specifically bind to individual cell types with high affinity. MREs are selected from initial large libraries of different nucleic or amino acids. This review outlines the construction of peptide and antibody fragment libraries as well as their different host types. Common methods of selection are also reviewed. Additionally, examples of cancer cell MREs are discussed, as well as their potential applications.
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spelling pubmed-45760122015-10-04 In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries Williams, Ryan M. Sooter, Letha J. J Immunol Res Review Article Differential cell systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro selection method for obtaining molecular recognition elements (MREs) that specifically bind to individual cell types with high affinity. MREs are selected from initial large libraries of different nucleic or amino acids. This review outlines the construction of peptide and antibody fragment libraries as well as their different host types. Common methods of selection are also reviewed. Additionally, examples of cancer cell MREs are discussed, as well as their potential applications. Hindawi Publishing Corporation 2015 2015-09-07 /pmc/articles/PMC4576012/ /pubmed/26436100 http://dx.doi.org/10.1155/2015/186586 Text en Copyright © 2015 R. M. Williams and L. J. Sooter. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Williams, Ryan M.
Sooter, Letha J.
In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries
title In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries
title_full In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries
title_fullStr In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries
title_full_unstemmed In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries
title_short In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries
title_sort in vitro selection of cancer cell-specific molecular recognition elements from amino acid libraries
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576012/
https://www.ncbi.nlm.nih.gov/pubmed/26436100
http://dx.doi.org/10.1155/2015/186586
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