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Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays

Pseudomonas aeruginosa is one of the Multi-Drug-Resistant organisms most frequently isolated worldwide and, because of a shortage of new antibiotics, bacteriophages are considered an alternative for its treatment. Previously, P. aeruginosa phages were isolated and best candidates were chosen based o...

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Autores principales: Holguín, Angela V., Rangel, Guillermo, Clavijo, Viviana, Prada, Catalina, Mantilla, Marcela, Gomez, María Catalina, Kutter, Elizabeth, Taylor, Corinda, Fineran, Peter C., Barrios, Andrés Fernando González, Vives, Martha J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576196/
https://www.ncbi.nlm.nih.gov/pubmed/26274971
http://dx.doi.org/10.3390/v7082835
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author Holguín, Angela V.
Rangel, Guillermo
Clavijo, Viviana
Prada, Catalina
Mantilla, Marcela
Gomez, María Catalina
Kutter, Elizabeth
Taylor, Corinda
Fineran, Peter C.
Barrios, Andrés Fernando González
Vives, Martha J.
author_facet Holguín, Angela V.
Rangel, Guillermo
Clavijo, Viviana
Prada, Catalina
Mantilla, Marcela
Gomez, María Catalina
Kutter, Elizabeth
Taylor, Corinda
Fineran, Peter C.
Barrios, Andrés Fernando González
Vives, Martha J.
author_sort Holguín, Angela V.
collection PubMed
description Pseudomonas aeruginosa is one of the Multi-Drug-Resistant organisms most frequently isolated worldwide and, because of a shortage of new antibiotics, bacteriophages are considered an alternative for its treatment. Previously, P. aeruginosa phages were isolated and best candidates were chosen based on their ability to form clear plaques and their host range. This work aimed to characterize one of those phages, ΦPan70, preliminarily identified as a good candidate for phage-therapy. We performed infection curves, biofilm removal assays, transmission-electron-microscopy, pulsed-field-gel-electrophoresis, and studied the in vivo ΦPan70 biological activity in the burned mouse model. ΦPan70 was classified as a member of the Myoviridae family and, in both planktonic cells and biofilms, was responsible for a significant reduction in the bacterial population. The burned mouse model showed an animal survival between 80% and 100%, significantly different from the control animals (0%). However, analysis of the ΦPan70 genome revealed that it was 64% identical to F10, a temperate P. aeruginosa phage. Gene annotation indicated ΦPan70 as a new, but possible temperate phage, therefore not ideal for phage-therapy. Based on this, we recommend genome sequence analysis as an early step to select candidate phages for potential application in phage-therapy, before entering into a more intensive characterization.
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spelling pubmed-45761962015-09-28 Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays Holguín, Angela V. Rangel, Guillermo Clavijo, Viviana Prada, Catalina Mantilla, Marcela Gomez, María Catalina Kutter, Elizabeth Taylor, Corinda Fineran, Peter C. Barrios, Andrés Fernando González Vives, Martha J. Viruses Article Pseudomonas aeruginosa is one of the Multi-Drug-Resistant organisms most frequently isolated worldwide and, because of a shortage of new antibiotics, bacteriophages are considered an alternative for its treatment. Previously, P. aeruginosa phages were isolated and best candidates were chosen based on their ability to form clear plaques and their host range. This work aimed to characterize one of those phages, ΦPan70, preliminarily identified as a good candidate for phage-therapy. We performed infection curves, biofilm removal assays, transmission-electron-microscopy, pulsed-field-gel-electrophoresis, and studied the in vivo ΦPan70 biological activity in the burned mouse model. ΦPan70 was classified as a member of the Myoviridae family and, in both planktonic cells and biofilms, was responsible for a significant reduction in the bacterial population. The burned mouse model showed an animal survival between 80% and 100%, significantly different from the control animals (0%). However, analysis of the ΦPan70 genome revealed that it was 64% identical to F10, a temperate P. aeruginosa phage. Gene annotation indicated ΦPan70 as a new, but possible temperate phage, therefore not ideal for phage-therapy. Based on this, we recommend genome sequence analysis as an early step to select candidate phages for potential application in phage-therapy, before entering into a more intensive characterization. MDPI 2015-08-12 /pmc/articles/PMC4576196/ /pubmed/26274971 http://dx.doi.org/10.3390/v7082835 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Holguín, Angela V.
Rangel, Guillermo
Clavijo, Viviana
Prada, Catalina
Mantilla, Marcela
Gomez, María Catalina
Kutter, Elizabeth
Taylor, Corinda
Fineran, Peter C.
Barrios, Andrés Fernando González
Vives, Martha J.
Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays
title Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays
title_full Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays
title_fullStr Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays
title_full_unstemmed Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays
title_short Phage ΦPan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays
title_sort phage φpan70, a putative temperate phage, controls pseudomonas aeruginosa in planktonic, biofilm and burn mouse model assays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576196/
https://www.ncbi.nlm.nih.gov/pubmed/26274971
http://dx.doi.org/10.3390/v7082835
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