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Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood

A specific humoral response to bacteriophages may follow phage application for medical purposes, and it may further determine the success or failure of the approach itself. We present a long-term study of antibody induction in mice by T4 phage applied per os: 100 days of phage treatment followed by...

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Autores principales: Majewska, Joanna, Beta, Weronika, Lecion, Dorota, Hodyra-Stefaniak, Katarzyna, Kłopot, Anna, Kaźmierczak, Zuzanna, Miernikiewicz, Paulina, Piotrowicz, Agnieszka, Ciekot, Jarosław, Owczarek, Barbara, Kopciuch, Agnieszka, Wojtyna, Karolina, Harhala, Marek, Mąkosa, Mateusz, Dąbrowska, Krystyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576206/
https://www.ncbi.nlm.nih.gov/pubmed/26308042
http://dx.doi.org/10.3390/v7082845
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author Majewska, Joanna
Beta, Weronika
Lecion, Dorota
Hodyra-Stefaniak, Katarzyna
Kłopot, Anna
Kaźmierczak, Zuzanna
Miernikiewicz, Paulina
Piotrowicz, Agnieszka
Ciekot, Jarosław
Owczarek, Barbara
Kopciuch, Agnieszka
Wojtyna, Karolina
Harhala, Marek
Mąkosa, Mateusz
Dąbrowska, Krystyna
author_facet Majewska, Joanna
Beta, Weronika
Lecion, Dorota
Hodyra-Stefaniak, Katarzyna
Kłopot, Anna
Kaźmierczak, Zuzanna
Miernikiewicz, Paulina
Piotrowicz, Agnieszka
Ciekot, Jarosław
Owczarek, Barbara
Kopciuch, Agnieszka
Wojtyna, Karolina
Harhala, Marek
Mąkosa, Mateusz
Dąbrowska, Krystyna
author_sort Majewska, Joanna
collection PubMed
description A specific humoral response to bacteriophages may follow phage application for medical purposes, and it may further determine the success or failure of the approach itself. We present a long-term study of antibody induction in mice by T4 phage applied per os: 100 days of phage treatment followed by 112 days without the phage, and subsequent second application of phage up to day 240. Serum and gut antibodies (IgM, IgG, secretory IgA) were analyzed in relation to microbiological status of the animals. T4 phage applied orally induced anti-phage antibodies when the exposure was long enough (IgG day 36, IgA day 79); the effect was related to high dosage. Termination of phage treatment resulted in a decrease of IgA again to insignificant levels. Second administration of phage induces secretory IgA sooner than that induced by the first administrations. Increased IgA level antagonized gut transit of active phage. Phage resistant E. coli dominated gut flora very late, on day 92. Thus, the immunological response emerges as a major factor determining phage survival in the gut. Phage proteins Hoc and gp12 were identified as highly immunogenic. A low response to exemplary foreign antigens (from Ebola virus) presented on Hoc was observed, which suggests that phage platforms can be used in oral vaccine design.
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spelling pubmed-45762062015-09-28 Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood Majewska, Joanna Beta, Weronika Lecion, Dorota Hodyra-Stefaniak, Katarzyna Kłopot, Anna Kaźmierczak, Zuzanna Miernikiewicz, Paulina Piotrowicz, Agnieszka Ciekot, Jarosław Owczarek, Barbara Kopciuch, Agnieszka Wojtyna, Karolina Harhala, Marek Mąkosa, Mateusz Dąbrowska, Krystyna Viruses Article A specific humoral response to bacteriophages may follow phage application for medical purposes, and it may further determine the success or failure of the approach itself. We present a long-term study of antibody induction in mice by T4 phage applied per os: 100 days of phage treatment followed by 112 days without the phage, and subsequent second application of phage up to day 240. Serum and gut antibodies (IgM, IgG, secretory IgA) were analyzed in relation to microbiological status of the animals. T4 phage applied orally induced anti-phage antibodies when the exposure was long enough (IgG day 36, IgA day 79); the effect was related to high dosage. Termination of phage treatment resulted in a decrease of IgA again to insignificant levels. Second administration of phage induces secretory IgA sooner than that induced by the first administrations. Increased IgA level antagonized gut transit of active phage. Phage resistant E. coli dominated gut flora very late, on day 92. Thus, the immunological response emerges as a major factor determining phage survival in the gut. Phage proteins Hoc and gp12 were identified as highly immunogenic. A low response to exemplary foreign antigens (from Ebola virus) presented on Hoc was observed, which suggests that phage platforms can be used in oral vaccine design. MDPI 2015-08-20 /pmc/articles/PMC4576206/ /pubmed/26308042 http://dx.doi.org/10.3390/v7082845 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Majewska, Joanna
Beta, Weronika
Lecion, Dorota
Hodyra-Stefaniak, Katarzyna
Kłopot, Anna
Kaźmierczak, Zuzanna
Miernikiewicz, Paulina
Piotrowicz, Agnieszka
Ciekot, Jarosław
Owczarek, Barbara
Kopciuch, Agnieszka
Wojtyna, Karolina
Harhala, Marek
Mąkosa, Mateusz
Dąbrowska, Krystyna
Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood
title Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood
title_full Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood
title_fullStr Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood
title_full_unstemmed Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood
title_short Oral Application of T4 Phage Induces Weak Antibody Production in the Gut and in the Blood
title_sort oral application of t4 phage induces weak antibody production in the gut and in the blood
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576206/
https://www.ncbi.nlm.nih.gov/pubmed/26308042
http://dx.doi.org/10.3390/v7082845
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