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Evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis
Mycobacterium tuberculosis (Mtb) infection remains one of the world’s major causes of illness and mortality. A clear understanding of the host defense against Mtb is imperatively needed forthe control of this epidemic. When tuberculosis (TB) infection occurs, a variety of pro and anti-inflammatory c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iuliu Hatieganu University of Medicine and Pharmacy
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576778/ https://www.ncbi.nlm.nih.gov/pubmed/26528066 http://dx.doi.org/10.15386/cjmed-459 |
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author | NDISHIMYE, PACIFIQUE SEGHROUCHNI, FOUAD DOMOKOS, BIANKA SORITAU, OLGA SADAK, ABDERRAHIM HOMORODEAN, DANIELA AOUADA, RAJAE EL POP, CARMEN MONICA |
author_facet | NDISHIMYE, PACIFIQUE SEGHROUCHNI, FOUAD DOMOKOS, BIANKA SORITAU, OLGA SADAK, ABDERRAHIM HOMORODEAN, DANIELA AOUADA, RAJAE EL POP, CARMEN MONICA |
author_sort | NDISHIMYE, PACIFIQUE |
collection | PubMed |
description | Mycobacterium tuberculosis (Mtb) infection remains one of the world’s major causes of illness and mortality. A clear understanding of the host defense against Mtb is imperatively needed forthe control of this epidemic. When tuberculosis (TB) infection occurs, a variety of pro and anti-inflammatory cytokines play a vital role in the pathogenesis of this disease. Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokines reported to suppress the protective immune response against tuberculosis. AIM: The aim of the present study was to evaluate the association of plasma IL-10 levels with various disease stages of TB and the possible effects of treatment on these levels. MATERIALS AND METHODS: A group of 30 patients with active pulmonary TB and a control group of 21 healthy individuals were enrolled in this study. The levels of IL-10 were measured before, during, and after treatment using commercially available enzyme-linked immune-sorbent assay (ELISA). Data were analyzed using GraphPad Prism version 5.0. RESULTS: The results showed that the levels of IL-10 had significant differences between the TB and control groups (p<0.05). The patients with abnormal chest X-Ray findings had higher IL-10 levels when compared to patients with normal X-Rays (p=0.03). A subgroup of 18 patients were followed during the treatment and the mean plasma concentration of IL-10 in patients before therapy was higher than in patients at 3 months of therapy and in patients after 6 months of therapy (p=0.01). However, the IL-10 level remained significantly higher in patients at the end of treatment compared with controls. These findings could be used in follow-up as clinical biomarker of the success of tuberculosis therapy. |
format | Online Article Text |
id | pubmed-4576778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Iuliu Hatieganu University of Medicine and Pharmacy |
record_format | MEDLINE/PubMed |
spelling | pubmed-45767782015-11-02 Evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis NDISHIMYE, PACIFIQUE SEGHROUCHNI, FOUAD DOMOKOS, BIANKA SORITAU, OLGA SADAK, ABDERRAHIM HOMORODEAN, DANIELA AOUADA, RAJAE EL POP, CARMEN MONICA Clujul Med Original Research Mycobacterium tuberculosis (Mtb) infection remains one of the world’s major causes of illness and mortality. A clear understanding of the host defense against Mtb is imperatively needed forthe control of this epidemic. When tuberculosis (TB) infection occurs, a variety of pro and anti-inflammatory cytokines play a vital role in the pathogenesis of this disease. Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokines reported to suppress the protective immune response against tuberculosis. AIM: The aim of the present study was to evaluate the association of plasma IL-10 levels with various disease stages of TB and the possible effects of treatment on these levels. MATERIALS AND METHODS: A group of 30 patients with active pulmonary TB and a control group of 21 healthy individuals were enrolled in this study. The levels of IL-10 were measured before, during, and after treatment using commercially available enzyme-linked immune-sorbent assay (ELISA). Data were analyzed using GraphPad Prism version 5.0. RESULTS: The results showed that the levels of IL-10 had significant differences between the TB and control groups (p<0.05). The patients with abnormal chest X-Ray findings had higher IL-10 levels when compared to patients with normal X-Rays (p=0.03). A subgroup of 18 patients were followed during the treatment and the mean plasma concentration of IL-10 in patients before therapy was higher than in patients at 3 months of therapy and in patients after 6 months of therapy (p=0.01). However, the IL-10 level remained significantly higher in patients at the end of treatment compared with controls. These findings could be used in follow-up as clinical biomarker of the success of tuberculosis therapy. Iuliu Hatieganu University of Medicine and Pharmacy 2015 2015-04-15 /pmc/articles/PMC4576778/ /pubmed/26528066 http://dx.doi.org/10.15386/cjmed-459 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License |
spellingShingle | Original Research NDISHIMYE, PACIFIQUE SEGHROUCHNI, FOUAD DOMOKOS, BIANKA SORITAU, OLGA SADAK, ABDERRAHIM HOMORODEAN, DANIELA AOUADA, RAJAE EL POP, CARMEN MONICA Evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis |
title | Evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis |
title_full | Evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis |
title_fullStr | Evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis |
title_full_unstemmed | Evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis |
title_short | Evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis |
title_sort | evaluation of interleukin-10 levels in the plasma of patients with various stages of tuberculosis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576778/ https://www.ncbi.nlm.nih.gov/pubmed/26528066 http://dx.doi.org/10.15386/cjmed-459 |
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