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Rapid but mild genoprotective effect on lymphocytic DNA with Panax notoginseng extract supplementation

BACKGROUND: Panax notoginseng (PN) is a well-known Chinese medicinal herb traditionally used as a hemostatic agent that strengthens and builds blood. The free radicals scavenging and antioxidant property of PN have been demonstrated in various studies either in vitro or in animal models, however, th...

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Autores principales: Szeto, Yim Tong, Lee, Loretta Kit Yee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGEYA 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576809/
https://www.ncbi.nlm.nih.gov/pubmed/26401366
http://dx.doi.org/10.5455/jice.20140819030913
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author Szeto, Yim Tong
Lee, Loretta Kit Yee
author_facet Szeto, Yim Tong
Lee, Loretta Kit Yee
author_sort Szeto, Yim Tong
collection PubMed
description BACKGROUND: Panax notoginseng (PN) is a well-known Chinese medicinal herb traditionally used as a hemostatic agent that strengthens and builds blood. The free radicals scavenging and antioxidant property of PN have been demonstrated in various studies either in vitro or in animal models, however, the genoprotective effect at human cellular level remains to be elucidated. AIM: The current supplementation study aimed to investigate the genoprotective effect of PN. The study explored the DNA protection effect after a single dose of 2500 mg commercial notoginseng extract in water. MATERIALS AND METHODS: Six subjects, (3 males, 3 females) were recruited and each attended two trials. In the first trial, pre-ingestion and 2 hour-post-ingestion blood samples were collected and they were challenged with 50 µM H2O2. In the second trial, water was taken instead as control. Lymphocytes with or without challenge were then subjected to comet assay. DNA damage was assessed under fluorescent microscope. RESULTS: Results showed a significant (P < 0.05) but mild decrease (3%) in the comet score after PN supplementation, indicating that PN supplementation reduces the H2O2-induced DNA damage in the lymphocytes and enhanced their resistance to oxidative damage giving a mild acute genoprotective effect against oxidant challenge. No change of comet score was observed in control trial.
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spelling pubmed-45768092015-09-23 Rapid but mild genoprotective effect on lymphocytic DNA with Panax notoginseng extract supplementation Szeto, Yim Tong Lee, Loretta Kit Yee J Intercult Ethnopharmacol Original Article BACKGROUND: Panax notoginseng (PN) is a well-known Chinese medicinal herb traditionally used as a hemostatic agent that strengthens and builds blood. The free radicals scavenging and antioxidant property of PN have been demonstrated in various studies either in vitro or in animal models, however, the genoprotective effect at human cellular level remains to be elucidated. AIM: The current supplementation study aimed to investigate the genoprotective effect of PN. The study explored the DNA protection effect after a single dose of 2500 mg commercial notoginseng extract in water. MATERIALS AND METHODS: Six subjects, (3 males, 3 females) were recruited and each attended two trials. In the first trial, pre-ingestion and 2 hour-post-ingestion blood samples were collected and they were challenged with 50 µM H2O2. In the second trial, water was taken instead as control. Lymphocytes with or without challenge were then subjected to comet assay. DNA damage was assessed under fluorescent microscope. RESULTS: Results showed a significant (P < 0.05) but mild decrease (3%) in the comet score after PN supplementation, indicating that PN supplementation reduces the H2O2-induced DNA damage in the lymphocytes and enhanced their resistance to oxidative damage giving a mild acute genoprotective effect against oxidant challenge. No change of comet score was observed in control trial. SAGEYA 2014-08-26 /pmc/articles/PMC4576809/ /pubmed/26401366 http://dx.doi.org/10.5455/jice.20140819030913 Text en Copyright: © SAGEYA http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, noncommercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Original Article
Szeto, Yim Tong
Lee, Loretta Kit Yee
Rapid but mild genoprotective effect on lymphocytic DNA with Panax notoginseng extract supplementation
title Rapid but mild genoprotective effect on lymphocytic DNA with Panax notoginseng extract supplementation
title_full Rapid but mild genoprotective effect on lymphocytic DNA with Panax notoginseng extract supplementation
title_fullStr Rapid but mild genoprotective effect on lymphocytic DNA with Panax notoginseng extract supplementation
title_full_unstemmed Rapid but mild genoprotective effect on lymphocytic DNA with Panax notoginseng extract supplementation
title_short Rapid but mild genoprotective effect on lymphocytic DNA with Panax notoginseng extract supplementation
title_sort rapid but mild genoprotective effect on lymphocytic dna with panax notoginseng extract supplementation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576809/
https://www.ncbi.nlm.nih.gov/pubmed/26401366
http://dx.doi.org/10.5455/jice.20140819030913
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