Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease

Mutation of the tobacco etch virus (TEV) protease nucleophile from cysteine to serine causes an approximately ∼10(4)-fold loss in activity. Ten rounds of directed evolution of the mutant, TEV(Ser), overcame the detrimental effects of nucleophile exchange to recover near-wild-type activity in the mut...

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Detalles Bibliográficos
Autores principales: Shafee, Thomas, Gatti-Lafranconi, Pietro, Minter, Ralph, Hollfelder, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2015
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576821/
https://www.ncbi.nlm.nih.gov/pubmed/26097079
http://dx.doi.org/10.1002/cbic.201500295

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576821/
https://www.ncbi.nlm.nih.gov/pubmed/26097079
http://dx.doi.org/10.1002/cbic.201500295

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