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Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease
Mutation of the tobacco etch virus (TEV) protease nucleophile from cysteine to serine causes an approximately ∼10(4)-fold loss in activity. Ten rounds of directed evolution of the mutant, TEV(Ser), overcame the detrimental effects of nucleophile exchange to recover near-wild-type activity in the mut...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
WILEY-VCH Verlag
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576821/ https://www.ncbi.nlm.nih.gov/pubmed/26097079 http://dx.doi.org/10.1002/cbic.201500295 |