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Complete Proton Transfer Cycle in GFP and Its T203V and S205V Mutants**

Proton transfer is critical in many important biochemical reactions. The unique three-step excited-state proton transfer in avGFP allows observations of protein proton transport in real-time. In this work we exploit femtosecond to microsecond transient IR spectroscopy to record, in D(2)O, the comple...

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Detalles Bibliográficos
Autores principales: Laptenok, Sergey P, Lukacs, Andras, Gil, Agnieszka, Brust, Richard, Sazanovich, Igor V, Greetham, Gregory M, Tonge, Peter J, Meech, Stephen R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576823/
https://www.ncbi.nlm.nih.gov/pubmed/26087935
http://dx.doi.org/10.1002/anie.201503672