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Complete Proton Transfer Cycle in GFP and Its T203V and S205V Mutants**
Proton transfer is critical in many important biochemical reactions. The unique three-step excited-state proton transfer in avGFP allows observations of protein proton transport in real-time. In this work we exploit femtosecond to microsecond transient IR spectroscopy to record, in D(2)O, the comple...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY-VCH Verlag
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576823/ https://www.ncbi.nlm.nih.gov/pubmed/26087935 http://dx.doi.org/10.1002/anie.201503672 |