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miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells

BACKGROUND: Although Helicobacter pylori infection is necessary for development of gastric adenocarcinoma (GAC), the underlying mechanism remains poorly defined. This study aimed to explore how miR-221 and miR-222 are dysregulated after H. pylori infection and how these 2 miRNAs are involved in path...

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Autores principales: Liu, Wenneng, Song, Nian, Yao, Huihua, Zhao, Liying, Liu, Hao, Li, Guoxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576921/
https://www.ncbi.nlm.nih.gov/pubmed/26364844
http://dx.doi.org/10.12659/MSM.894324
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author Liu, Wenneng
Song, Nian
Yao, Huihua
Zhao, Liying
Liu, Hao
Li, Guoxin
author_facet Liu, Wenneng
Song, Nian
Yao, Huihua
Zhao, Liying
Liu, Hao
Li, Guoxin
author_sort Liu, Wenneng
collection PubMed
description BACKGROUND: Although Helicobacter pylori infection is necessary for development of gastric adenocarcinoma (GAC), the underlying mechanism remains poorly defined. This study aimed to explore how miR-221 and miR-222 are dysregulated after H. pylori infection and how these 2 miRNAs are involved in pathological development of gastric cancer. MATERIAL/METHODS: qRT-PCR analysis was performed to quantify miR-221 and miR-222 expression in patients with H. pylori – induced chronic gastritis, H. pylori-negative healthy controls, and in gastric cancer tissues and the corresponding adjacent normal tissues. Cell models were used to verify the expression profile. Dual luciferase assay was performed to verify putative binding between miR-221 or miR-222 and RECK. A loss-and-gain function study was performed to assess the miR-221/miR-222-RECK axis in gastric cancer cells. RESULTS: H. pylori infection leads to significantly higher miR-221 and miR-222 expression. MiR-221 and miR-222 can bind the same sequence of RECK 3′UTR, thereby modulating its expression. Through simultaneous regulation over RECK, miR-221 and miR-222 can promote gastric cancer cell growth and invasion. CONCLUSIONS: The miR-221/miR-222-RECK axis might be an important path modulating H. pylori infection-related gastric cancer development.
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spelling pubmed-45769212015-09-30 miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells Liu, Wenneng Song, Nian Yao, Huihua Zhao, Liying Liu, Hao Li, Guoxin Med Sci Monit Molecular Biology BACKGROUND: Although Helicobacter pylori infection is necessary for development of gastric adenocarcinoma (GAC), the underlying mechanism remains poorly defined. This study aimed to explore how miR-221 and miR-222 are dysregulated after H. pylori infection and how these 2 miRNAs are involved in pathological development of gastric cancer. MATERIAL/METHODS: qRT-PCR analysis was performed to quantify miR-221 and miR-222 expression in patients with H. pylori – induced chronic gastritis, H. pylori-negative healthy controls, and in gastric cancer tissues and the corresponding adjacent normal tissues. Cell models were used to verify the expression profile. Dual luciferase assay was performed to verify putative binding between miR-221 or miR-222 and RECK. A loss-and-gain function study was performed to assess the miR-221/miR-222-RECK axis in gastric cancer cells. RESULTS: H. pylori infection leads to significantly higher miR-221 and miR-222 expression. MiR-221 and miR-222 can bind the same sequence of RECK 3′UTR, thereby modulating its expression. Through simultaneous regulation over RECK, miR-221 and miR-222 can promote gastric cancer cell growth and invasion. CONCLUSIONS: The miR-221/miR-222-RECK axis might be an important path modulating H. pylori infection-related gastric cancer development. International Scientific Literature, Inc. 2015-09-13 /pmc/articles/PMC4576921/ /pubmed/26364844 http://dx.doi.org/10.12659/MSM.894324 Text en © Med Sci Monit, 2015 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License
spellingShingle Molecular Biology
Liu, Wenneng
Song, Nian
Yao, Huihua
Zhao, Liying
Liu, Hao
Li, Guoxin
miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells
title miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells
title_full miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells
title_fullStr miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells
title_full_unstemmed miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells
title_short miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells
title_sort mir-221 and mir-222 simultaneously target reck and regulate growth and invasion of gastric cancer cells
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576921/
https://www.ncbi.nlm.nih.gov/pubmed/26364844
http://dx.doi.org/10.12659/MSM.894324
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