Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq

The brain is an organ that consists of various cell types. As our knowledge of the structure and function of the brain progresses, cell type-specific research is gaining importance. Together with advances in sequencing technology and bioinformatics, cell type-specific transcriptome studies are provi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, TaeHyun, Lim, Chae-Seok, Kaang, Bong-Kiun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2015
Materias:
Contributed Mini Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577288/
https://www.ncbi.nlm.nih.gov/pubmed/25603796
http://dx.doi.org/10.5483/BMBRep.2015.48.7.218
_version_ 1782390964506066944
author Kim, TaeHyun
Lim, Chae-Seok
Kaang, Bong-Kiun
author_facet Kim, TaeHyun
Lim, Chae-Seok
Kaang, Bong-Kiun
author_sort Kim, TaeHyun
collection PubMed
description The brain is an organ that consists of various cell types. As our knowledge of the structure and function of the brain progresses, cell type-specific research is gaining importance. Together with advances in sequencing technology and bioinformatics, cell type-specific transcriptome studies are providing important insights into brain cell function. In this review, we discuss 3 different cell type-specific transcriptome analyses i.e., Laser Capture Microdissection (LCM), Translating Ribosome Affinity Purification (TRAP)/RiboTag, and single cell RNA-Seq, that are widely used in the field of neuroscience. [BMB Reports 2015; 48(7): 388-394]
format Online
Article
Text
id pubmed-4577288
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Korean Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-45772882015-09-22 Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq Kim, TaeHyun Lim, Chae-Seok Kaang, Bong-Kiun BMB Rep Contributed Mini Review The brain is an organ that consists of various cell types. As our knowledge of the structure and function of the brain progresses, cell type-specific research is gaining importance. Together with advances in sequencing technology and bioinformatics, cell type-specific transcriptome studies are providing important insights into brain cell function. In this review, we discuss 3 different cell type-specific transcriptome analyses i.e., Laser Capture Microdissection (LCM), Translating Ribosome Affinity Purification (TRAP)/RiboTag, and single cell RNA-Seq, that are widely used in the field of neuroscience. [BMB Reports 2015; 48(7): 388-394] Korean Society for Biochemistry and Molecular Biology 2015-07-31 /pmc/articles/PMC4577288/ /pubmed/25603796 http://dx.doi.org/10.5483/BMBRep.2015.48.7.218 Text en Copyright © 2015, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Contributed Mini Review
Kim, TaeHyun
Lim, Chae-Seok
Kaang, Bong-Kiun
Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq
title Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq
title_full Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq
title_fullStr Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq
title_full_unstemmed Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq
title_short Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq
title_sort cell type-specific gene expression profiling in brain tissue: comparison between trap, lcm and rna-seq
topic Contributed Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577288/
https://www.ncbi.nlm.nih.gov/pubmed/25603796
http://dx.doi.org/10.5483/BMBRep.2015.48.7.218
work_keys_str_mv AT kimtaehyun celltypespecificgeneexpressionprofilinginbraintissuecomparisonbetweentraplcmandrnaseq
AT limchaeseok celltypespecificgeneexpressionprofilinginbraintissuecomparisonbetweentraplcmandrnaseq
AT kaangbongkiun celltypespecificgeneexpressionprofilinginbraintissuecomparisonbetweentraplcmandrnaseq

Ejemplares similares