Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B

Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction wi...

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Detalles Bibliográficos
Autores principales: Han, Arim, Chae, Yun-Cheol, Park, Jin Woo, Kim, Kee-Beom, Kim, Ji-Young, Seo, Sang-Beom
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2015
Materias:
Research-Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577290/
https://www.ncbi.nlm.nih.gov/pubmed/25413303
http://dx.doi.org/10.5483/BMBRep.2015.48.7.188
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author Han, Arim
Chae, Yun-Cheol
Park, Jin Woo
Kim, Kee-Beom
Kim, Ji-Young
Seo, Sang-Beom
author_facet Han, Arim
Chae, Yun-Cheol
Park, Jin Woo
Kim, Kee-Beom
Kim, Ji-Young
Seo, Sang-Beom
author_sort Han, Arim
collection PubMed
description Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed at the promoter area of ANGPT1. Using MTT and wound healing assays, depletion of KDM3B was found to increase cell proliferation and cell motility, indicating that KDM3B has a role in angiogenesis. [BMB Reports 2015; 48(7): 401-406]
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spelling pubmed-45772902015-09-22 Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B Han, Arim Chae, Yun-Cheol Park, Jin Woo Kim, Kee-Beom Kim, Ji-Young Seo, Sang-Beom BMB Rep Research-Article Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed at the promoter area of ANGPT1. Using MTT and wound healing assays, depletion of KDM3B was found to increase cell proliferation and cell motility, indicating that KDM3B has a role in angiogenesis. [BMB Reports 2015; 48(7): 401-406] Korean Society for Biochemistry and Molecular Biology 2015-07-31 /pmc/articles/PMC4577290/ /pubmed/25413303 http://dx.doi.org/10.5483/BMBRep.2015.48.7.188 Text en Copyright © 2015, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research-Article
Han, Arim
Chae, Yun-Cheol
Park, Jin Woo
Kim, Kee-Beom
Kim, Ji-Young
Seo, Sang-Beom
Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B
title Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B
title_full Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B
title_fullStr Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B
title_full_unstemmed Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B
title_short Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B
title_sort transcriptional repression of angpt1 by histone h3k9 demethylase kdm3b
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577290/
https://www.ncbi.nlm.nih.gov/pubmed/25413303
http://dx.doi.org/10.5483/BMBRep.2015.48.7.188
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