Effects of As(2)O(3) nanoparticles on cell growth and apoptosis of NB4 cells

The aim of the present study was to explore the preparation of arsenic trioxide (As(2)O(3)) nanoparticles and examine the antitumor effects of these nanoparticles on NB4 cells. As(2)O(3) nanoparticles were prepared using the sol-gel method and characterized using transmission electron microscopy and energy dispersive spectroscopy. The results indicated that the As(2)O(3) nanoparticles prepared in the present study were round or elliptical, well dispersed and had an ~40-nm or <10-nm diameter. The antitumor effects of As(2)O(3) nanoparticles at various concentrations were analyzed by flow cytometry and the MTT assay, and were compared with those of traditional As(2)O(3) solution. At the same concentration and incubation time (48 h), the survival rate of cells treated with As(2)O(3) nanoparticles was significantly lower than that of cells treated with the As(2)O(3) solution. The growth inhibition rate under both treatments was time- and dose-dependent. In addition, at the same concentration and incubation time, the apoptosis rate of the cells treated with As(2)O(3) nanoparticles was significantly higher than that of the cells treated with the As(2)O(3) solution. Furthermore, As(2)O(3) nanoparticles resulted in a greater reduction in the expression of the anti-apoptotic protein B-cell lymphoma 2 compared with the As(2)O(3) solution. In conclusion, As(2)O(3) nanoparticles, prepared using the sol-gel method, were found to produce a stronger cytotoxic effect on tumor cells than that produced by the As(2)O(3) solution, possibly by inhibiting Bcl-2 expression.