Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)

PREMISE OF THE STUDY: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). METHODS AND RESULTS: Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interp...

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Detalles Bibliográficos
Autores principales: Müller, Eike, Hlaváčková, Iva, Svoen, Mildrid Elvik, Alsos, Inger Greve, Eidesen, Pernille Bronken
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578374/
https://www.ncbi.nlm.nih.gov/pubmed/26421249
http://dx.doi.org/10.3732/apps.1500036
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author Müller, Eike
Hlaváčková, Iva
Svoen, Mildrid Elvik
Alsos, Inger Greve
Eidesen, Pernille Bronken
author_facet Müller, Eike
Hlaváčková, Iva
Svoen, Mildrid Elvik
Alsos, Inger Greve
Eidesen, Pernille Bronken
author_sort Müller, Eike
collection PubMed
description PREMISE OF THE STUDY: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). METHODS AND RESULTS: Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosity per locus ranged from 0.06 to 0.68. Analysis of F(0) and F(1) samples proved that one allele was always inherited maternally. Four multiplex mixes have been developed. CONCLUSIONS: Microsatellite markers for this species will be a valuable tool to study detailed small-scale genetic patterns in an arctic/alpine herb and to relate them to demographic parameters.
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spelling pubmed-45783742015-09-29 Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1) Müller, Eike Hlaváčková, Iva Svoen, Mildrid Elvik Alsos, Inger Greve Eidesen, Pernille Bronken Appl Plant Sci Primer Note PREMISE OF THE STUDY: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). METHODS AND RESULTS: Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosity per locus ranged from 0.06 to 0.68. Analysis of F(0) and F(1) samples proved that one allele was always inherited maternally. Four multiplex mixes have been developed. CONCLUSIONS: Microsatellite markers for this species will be a valuable tool to study detailed small-scale genetic patterns in an arctic/alpine herb and to relate them to demographic parameters. Botanical Society of America 2015-09-08 /pmc/articles/PMC4578374/ /pubmed/26421249 http://dx.doi.org/10.3732/apps.1500036 Text en © 2015 Müller et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Müller, Eike
Hlaváčková, Iva
Svoen, Mildrid Elvik
Alsos, Inger Greve
Eidesen, Pernille Bronken
Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)
title Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)
title_full Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)
title_fullStr Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)
title_full_unstemmed Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)
title_short Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)
title_sort characterization of 14 microsatellite markers for silene acaulis (caryophyllaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578374/
https://www.ncbi.nlm.nih.gov/pubmed/26421249
http://dx.doi.org/10.3732/apps.1500036
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