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Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)(1)
PREMISE OF THE STUDY: Microsatellite loci were isolated in Hypolepis punctata (Dennstaedtiaceae) to further study the reproductive ecology of this species. METHODS AND RESULTS: We developed 16 microsatellite loci from one sample of H. punctata using an enriched genomic library. These loci were chara...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578377/ https://www.ncbi.nlm.nih.gov/pubmed/26421252 http://dx.doi.org/10.3732/apps.1500047 |
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author | Shang, Hui Wang, Ying Yan, Yue-Hong |
author_facet | Shang, Hui Wang, Ying Yan, Yue-Hong |
author_sort | Shang, Hui |
collection | PubMed |
description | PREMISE OF THE STUDY: Microsatellite loci were isolated in Hypolepis punctata (Dennstaedtiaceae) to further study the reproductive ecology of this species. METHODS AND RESULTS: We developed 16 microsatellite loci from one sample of H. punctata using an enriched genomic library. These loci were characterized in 28 individuals. The number of alleles per locus ranged from two to 10, and the expected heterozygosity ranged from 0.036 to 0.845. CONCLUSIONS: The results indicate that the microsatellite markers can facilitate further studies on inferring the phylogeography and population genetics of H. punctata and related species. |
format | Online Article Text |
id | pubmed-4578377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-45783772015-09-29 Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)(1) Shang, Hui Wang, Ying Yan, Yue-Hong Appl Plant Sci Primer Note PREMISE OF THE STUDY: Microsatellite loci were isolated in Hypolepis punctata (Dennstaedtiaceae) to further study the reproductive ecology of this species. METHODS AND RESULTS: We developed 16 microsatellite loci from one sample of H. punctata using an enriched genomic library. These loci were characterized in 28 individuals. The number of alleles per locus ranged from two to 10, and the expected heterozygosity ranged from 0.036 to 0.845. CONCLUSIONS: The results indicate that the microsatellite markers can facilitate further studies on inferring the phylogeography and population genetics of H. punctata and related species. Botanical Society of America 2015-09-04 /pmc/articles/PMC4578377/ /pubmed/26421252 http://dx.doi.org/10.3732/apps.1500047 Text en © 2015 Shang et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Shang, Hui Wang, Ying Yan, Yue-Hong Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)(1) |
title | Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)(1) |
title_full | Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)(1) |
title_fullStr | Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)(1) |
title_full_unstemmed | Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)(1) |
title_short | Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)(1) |
title_sort | development and characterization of microsatellite loci in the pantropical fern hypolepis punctata (dennstaedtiaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578377/ https://www.ncbi.nlm.nih.gov/pubmed/26421252 http://dx.doi.org/10.3732/apps.1500047 |
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