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Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1)
PREMISE OF THE STUDY: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS: Fifteen simple sequen...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578378/ https://www.ncbi.nlm.nih.gov/pubmed/26421253 http://dx.doi.org/10.3732/apps.1500049 |
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author | Gardner, Elliot M. Laricchia, Kristen M. Murphy, Matthew Ragone, Diane Scheffler, Brian E. Simpson, Sheron Williams, Evelyn W. Zerega, Nyree J. C. |
author_facet | Gardner, Elliot M. Laricchia, Kristen M. Murphy, Matthew Ragone, Diane Scheffler, Brian E. Simpson, Sheron Williams, Evelyn W. Zerega, Nyree J. C. |
author_sort | Gardner, Elliot M. |
collection | PubMed |
description | PREMISE OF THE STUDY: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS: Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis × A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy. CONCLUSIONS: These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit. |
format | Online Article Text |
id | pubmed-4578378 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-45783782015-09-29 Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1) Gardner, Elliot M. Laricchia, Kristen M. Murphy, Matthew Ragone, Diane Scheffler, Brian E. Simpson, Sheron Williams, Evelyn W. Zerega, Nyree J. C. Appl Plant Sci Primer Note PREMISE OF THE STUDY: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS: Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis × A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy. CONCLUSIONS: These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit. Botanical Society of America 2015-09-10 /pmc/articles/PMC4578378/ /pubmed/26421253 http://dx.doi.org/10.3732/apps.1500049 Text en © 2015 Gardner et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This article is a U.S. Government work and is in the public domain in the USA. |
spellingShingle | Primer Note Gardner, Elliot M. Laricchia, Kristen M. Murphy, Matthew Ragone, Diane Scheffler, Brian E. Simpson, Sheron Williams, Evelyn W. Zerega, Nyree J. C. Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1) |
title | Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1) |
title_full | Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1) |
title_fullStr | Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1) |
title_full_unstemmed | Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1) |
title_short | Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1) |
title_sort | chloroplast microsatellite markers for artocarpus (moraceae) developed from transcriptome sequences(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578378/ https://www.ncbi.nlm.nih.gov/pubmed/26421253 http://dx.doi.org/10.3732/apps.1500049 |
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