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Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1)

PREMISE OF THE STUDY: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS: Fifteen simple sequen...

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Autores principales: Gardner, Elliot M., Laricchia, Kristen M., Murphy, Matthew, Ragone, Diane, Scheffler, Brian E., Simpson, Sheron, Williams, Evelyn W., Zerega, Nyree J. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578378/
https://www.ncbi.nlm.nih.gov/pubmed/26421253
http://dx.doi.org/10.3732/apps.1500049
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author Gardner, Elliot M.
Laricchia, Kristen M.
Murphy, Matthew
Ragone, Diane
Scheffler, Brian E.
Simpson, Sheron
Williams, Evelyn W.
Zerega, Nyree J. C.
author_facet Gardner, Elliot M.
Laricchia, Kristen M.
Murphy, Matthew
Ragone, Diane
Scheffler, Brian E.
Simpson, Sheron
Williams, Evelyn W.
Zerega, Nyree J. C.
author_sort Gardner, Elliot M.
collection PubMed
description PREMISE OF THE STUDY: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS: Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis × A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy. CONCLUSIONS: These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit.
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spelling pubmed-45783782015-09-29 Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1) Gardner, Elliot M. Laricchia, Kristen M. Murphy, Matthew Ragone, Diane Scheffler, Brian E. Simpson, Sheron Williams, Evelyn W. Zerega, Nyree J. C. Appl Plant Sci Primer Note PREMISE OF THE STUDY: Chloroplast microsatellite loci were characterized from transcriptomes of Artocarpus altilis (breadfruit) and A. camansi (breadnut). They were tested in A. odoratissimus (terap) and A. altilis and evaluated in silico for two congeners. METHODS AND RESULTS: Fifteen simple sequence repeats (SSRs) were identified in chloroplast sequences from four Artocarpus transcriptome assemblies. The markers were evaluated using capillary electrophoresis in A. odoratissimus (105 accessions) and A. altilis (73). They were also evaluated in silico in A. altilis (10), A. camansi (6), and A. altilis × A. mariannensis (7) transcriptomes. All loci were polymorphic in at least one species, with all 15 polymorphic in A. camansi. Per species, average alleles per locus ranged between 2.2 and 2.5. Three loci had evidence of fragment-length homoplasy. CONCLUSIONS: These markers will complement existing nuclear markers by enabling confident identification of maternal and clone lines, which are often important in vegetatively propagated crops such as breadfruit. Botanical Society of America 2015-09-10 /pmc/articles/PMC4578378/ /pubmed/26421253 http://dx.doi.org/10.3732/apps.1500049 Text en © 2015 Gardner et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This article is a U.S. Government work and is in the public domain in the USA.
spellingShingle Primer Note
Gardner, Elliot M.
Laricchia, Kristen M.
Murphy, Matthew
Ragone, Diane
Scheffler, Brian E.
Simpson, Sheron
Williams, Evelyn W.
Zerega, Nyree J. C.
Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1)
title Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1)
title_full Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1)
title_fullStr Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1)
title_full_unstemmed Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1)
title_short Chloroplast microsatellite markers for Artocarpus (Moraceae) developed from transcriptome sequences(1)
title_sort chloroplast microsatellite markers for artocarpus (moraceae) developed from transcriptome sequences(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578378/
https://www.ncbi.nlm.nih.gov/pubmed/26421253
http://dx.doi.org/10.3732/apps.1500049
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