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Anticancer activity of CopA3 dimer peptide in human gastric cancer cells
CopA3 is a homodimeric α-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the ant...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Biochemistry and Molecular Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578618/ https://www.ncbi.nlm.nih.gov/pubmed/25047444 http://dx.doi.org/10.5483/BMBRep.2015.48.6.073 |
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author | Lee, Joon Ha Kim, In-Woo Kim, Sang-Hee Yun, Eun-Young Nam, Sung-Hee Ahn, Mi-Young Kang, Dong-Chul Hwang, Jae Sam |
author_facet | Lee, Joon Ha Kim, In-Woo Kim, Sang-Hee Yun, Eun-Young Nam, Sung-Hee Ahn, Mi-Young Kang, Dong-Chul Hwang, Jae Sam |
author_sort | Lee, Joon Ha |
collection | PubMed |
description | CopA3 is a homodimeric α-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the anticancer activity of CopA3 in human gastric cancer cells. CopA3 reduced cell viability and it was cytotoxic to gastric cancer cells in the MTS and LDH release assay, respectively. CopA3 was shown to induce necrotic cell death of the gastric cancer cells by flow cytometric analysis and acridine orange/ethidium bromide staining. CopA3-induced cell death was mediated by specific interactions with phosphatidylserine, a membrane component of cancer cells. Taken together, these data indicated that CopA3 mainly caused necrosis of gastric cancer cells, probably through interactions with phosphatidylserine, which suggests the potential utility of CopA3 as a cancer therapeutic. [BMB Reports 2015; 48(6): 324-329] |
format | Online Article Text |
id | pubmed-4578618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-45786182015-09-22 Anticancer activity of CopA3 dimer peptide in human gastric cancer cells Lee, Joon Ha Kim, In-Woo Kim, Sang-Hee Yun, Eun-Young Nam, Sung-Hee Ahn, Mi-Young Kang, Dong-Chul Hwang, Jae Sam BMB Rep Research-Articles CopA3 is a homodimeric α-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the anticancer activity of CopA3 in human gastric cancer cells. CopA3 reduced cell viability and it was cytotoxic to gastric cancer cells in the MTS and LDH release assay, respectively. CopA3 was shown to induce necrotic cell death of the gastric cancer cells by flow cytometric analysis and acridine orange/ethidium bromide staining. CopA3-induced cell death was mediated by specific interactions with phosphatidylserine, a membrane component of cancer cells. Taken together, these data indicated that CopA3 mainly caused necrosis of gastric cancer cells, probably through interactions with phosphatidylserine, which suggests the potential utility of CopA3 as a cancer therapeutic. [BMB Reports 2015; 48(6): 324-329] Korean Society for Biochemistry and Molecular Biology 2015-06-30 /pmc/articles/PMC4578618/ /pubmed/25047444 http://dx.doi.org/10.5483/BMBRep.2015.48.6.073 Text en Copyright © 2015, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research-Articles Lee, Joon Ha Kim, In-Woo Kim, Sang-Hee Yun, Eun-Young Nam, Sung-Hee Ahn, Mi-Young Kang, Dong-Chul Hwang, Jae Sam Anticancer activity of CopA3 dimer peptide in human gastric cancer cells |
title | Anticancer activity of CopA3 dimer peptide in human gastric cancer cells |
title_full | Anticancer activity of CopA3 dimer peptide in human gastric cancer cells |
title_fullStr | Anticancer activity of CopA3 dimer peptide in human gastric cancer cells |
title_full_unstemmed | Anticancer activity of CopA3 dimer peptide in human gastric cancer cells |
title_short | Anticancer activity of CopA3 dimer peptide in human gastric cancer cells |
title_sort | anticancer activity of copa3 dimer peptide in human gastric cancer cells |
topic | Research-Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578618/ https://www.ncbi.nlm.nih.gov/pubmed/25047444 http://dx.doi.org/10.5483/BMBRep.2015.48.6.073 |
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