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Anticancer activity of CopA3 dimer peptide in human gastric cancer cells

CopA3 is a homodimeric α-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the ant...

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Autores principales: Lee, Joon Ha, Kim, In-Woo, Kim, Sang-Hee, Yun, Eun-Young, Nam, Sung-Hee, Ahn, Mi-Young, Kang, Dong-Chul, Hwang, Jae Sam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578618/
https://www.ncbi.nlm.nih.gov/pubmed/25047444
http://dx.doi.org/10.5483/BMBRep.2015.48.6.073
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author Lee, Joon Ha
Kim, In-Woo
Kim, Sang-Hee
Yun, Eun-Young
Nam, Sung-Hee
Ahn, Mi-Young
Kang, Dong-Chul
Hwang, Jae Sam
author_facet Lee, Joon Ha
Kim, In-Woo
Kim, Sang-Hee
Yun, Eun-Young
Nam, Sung-Hee
Ahn, Mi-Young
Kang, Dong-Chul
Hwang, Jae Sam
author_sort Lee, Joon Ha
collection PubMed
description CopA3 is a homodimeric α-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the anticancer activity of CopA3 in human gastric cancer cells. CopA3 reduced cell viability and it was cytotoxic to gastric cancer cells in the MTS and LDH release assay, respectively. CopA3 was shown to induce necrotic cell death of the gastric cancer cells by flow cytometric analysis and acridine orange/ethidium bromide staining. CopA3-induced cell death was mediated by specific interactions with phosphatidylserine, a membrane component of cancer cells. Taken together, these data indicated that CopA3 mainly caused necrosis of gastric cancer cells, probably through interactions with phosphatidylserine, which suggests the potential utility of CopA3 as a cancer therapeutic. [BMB Reports 2015; 48(6): 324-329]
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spelling pubmed-45786182015-09-22 Anticancer activity of CopA3 dimer peptide in human gastric cancer cells Lee, Joon Ha Kim, In-Woo Kim, Sang-Hee Yun, Eun-Young Nam, Sung-Hee Ahn, Mi-Young Kang, Dong-Chul Hwang, Jae Sam BMB Rep Research-Articles CopA3 is a homodimeric α-helical peptide derived from coprisin which is a defensin-like antimicrobial peptide that was identified from the dung beetle, Copris tripartitus. CopA3 has been reported to have anticancer activity against leukemia cancer cells. In the present study, we investigated the anticancer activity of CopA3 in human gastric cancer cells. CopA3 reduced cell viability and it was cytotoxic to gastric cancer cells in the MTS and LDH release assay, respectively. CopA3 was shown to induce necrotic cell death of the gastric cancer cells by flow cytometric analysis and acridine orange/ethidium bromide staining. CopA3-induced cell death was mediated by specific interactions with phosphatidylserine, a membrane component of cancer cells. Taken together, these data indicated that CopA3 mainly caused necrosis of gastric cancer cells, probably through interactions with phosphatidylserine, which suggests the potential utility of CopA3 as a cancer therapeutic. [BMB Reports 2015; 48(6): 324-329] Korean Society for Biochemistry and Molecular Biology 2015-06-30 /pmc/articles/PMC4578618/ /pubmed/25047444 http://dx.doi.org/10.5483/BMBRep.2015.48.6.073 Text en Copyright © 2015, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research-Articles
Lee, Joon Ha
Kim, In-Woo
Kim, Sang-Hee
Yun, Eun-Young
Nam, Sung-Hee
Ahn, Mi-Young
Kang, Dong-Chul
Hwang, Jae Sam
Anticancer activity of CopA3 dimer peptide in human gastric cancer cells
title Anticancer activity of CopA3 dimer peptide in human gastric cancer cells
title_full Anticancer activity of CopA3 dimer peptide in human gastric cancer cells
title_fullStr Anticancer activity of CopA3 dimer peptide in human gastric cancer cells
title_full_unstemmed Anticancer activity of CopA3 dimer peptide in human gastric cancer cells
title_short Anticancer activity of CopA3 dimer peptide in human gastric cancer cells
title_sort anticancer activity of copa3 dimer peptide in human gastric cancer cells
topic Research-Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578618/
https://www.ncbi.nlm.nih.gov/pubmed/25047444
http://dx.doi.org/10.5483/BMBRep.2015.48.6.073
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