Cargando…

Global Identification of Genes Specific for Rice Meiosis

The leptotene-zygotene transition is a major step in meiotic progression during which pairing between homologous chromosomes is initiated and double strand breaks occur. OsAM1, a homologue of maize AM1 and Arabidopsis SWI1, encodes a protein with a coiled-coil domain in its central region that is re...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Bingwei, Xu, Meng, Bian, Shiquan, Hou, Lili, Tang, Ding, Li, Yafei, Gu, Minghong, Cheng, Zhukuan, Yu, Hengxiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578934/
https://www.ncbi.nlm.nih.gov/pubmed/26394329
http://dx.doi.org/10.1371/journal.pone.0137399
_version_ 1782391190069444608
author Zhang, Bingwei
Xu, Meng
Bian, Shiquan
Hou, Lili
Tang, Ding
Li, Yafei
Gu, Minghong
Cheng, Zhukuan
Yu, Hengxiu
author_facet Zhang, Bingwei
Xu, Meng
Bian, Shiquan
Hou, Lili
Tang, Ding
Li, Yafei
Gu, Minghong
Cheng, Zhukuan
Yu, Hengxiu
author_sort Zhang, Bingwei
collection PubMed
description The leptotene-zygotene transition is a major step in meiotic progression during which pairing between homologous chromosomes is initiated and double strand breaks occur. OsAM1, a homologue of maize AM1 and Arabidopsis SWI1, encodes a protein with a coiled-coil domain in its central region that is required for the leptotene-zygotene transition during rice meiosis. To gain more insight into the role of OsAM1 in rice meiosis and identify additional meiosis-specific genes, we characterized the transcriptomes of young panicles of Osam1 mutant and wild-type rice plants using RNA-Seq combined with bioinformatic and statistical analyses. As a result, a total of 25,750 and 28,455 genes were expressed in young panicles of wild-type and Osam1 mutant plants, respectively, and 4,400 differentially expressed genes (DEGs; log(2) Ratio ≥ 1, FDR ≤ 0.05) were identified. Of these DEGs, four known rice meiosis-specific genes were detected, and 22 new putative meiosis-related genes were found by mapping these DEGs to reference biological pathways in the KEGG database. We identified eight additional well-conserved OsAM1-responsive rice meiotic genes by comparing our RNA-Seq data with known meiotic genes in Arabidopsis and fission yeast.
format Online
Article
Text
id pubmed-4578934
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45789342015-10-01 Global Identification of Genes Specific for Rice Meiosis Zhang, Bingwei Xu, Meng Bian, Shiquan Hou, Lili Tang, Ding Li, Yafei Gu, Minghong Cheng, Zhukuan Yu, Hengxiu PLoS One Research Article The leptotene-zygotene transition is a major step in meiotic progression during which pairing between homologous chromosomes is initiated and double strand breaks occur. OsAM1, a homologue of maize AM1 and Arabidopsis SWI1, encodes a protein with a coiled-coil domain in its central region that is required for the leptotene-zygotene transition during rice meiosis. To gain more insight into the role of OsAM1 in rice meiosis and identify additional meiosis-specific genes, we characterized the transcriptomes of young panicles of Osam1 mutant and wild-type rice plants using RNA-Seq combined with bioinformatic and statistical analyses. As a result, a total of 25,750 and 28,455 genes were expressed in young panicles of wild-type and Osam1 mutant plants, respectively, and 4,400 differentially expressed genes (DEGs; log(2) Ratio ≥ 1, FDR ≤ 0.05) were identified. Of these DEGs, four known rice meiosis-specific genes were detected, and 22 new putative meiosis-related genes were found by mapping these DEGs to reference biological pathways in the KEGG database. We identified eight additional well-conserved OsAM1-responsive rice meiotic genes by comparing our RNA-Seq data with known meiotic genes in Arabidopsis and fission yeast. Public Library of Science 2015-09-22 /pmc/articles/PMC4578934/ /pubmed/26394329 http://dx.doi.org/10.1371/journal.pone.0137399 Text en © 2015 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Bingwei
Xu, Meng
Bian, Shiquan
Hou, Lili
Tang, Ding
Li, Yafei
Gu, Minghong
Cheng, Zhukuan
Yu, Hengxiu
Global Identification of Genes Specific for Rice Meiosis
title Global Identification of Genes Specific for Rice Meiosis
title_full Global Identification of Genes Specific for Rice Meiosis
title_fullStr Global Identification of Genes Specific for Rice Meiosis
title_full_unstemmed Global Identification of Genes Specific for Rice Meiosis
title_short Global Identification of Genes Specific for Rice Meiosis
title_sort global identification of genes specific for rice meiosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578934/
https://www.ncbi.nlm.nih.gov/pubmed/26394329
http://dx.doi.org/10.1371/journal.pone.0137399
work_keys_str_mv AT zhangbingwei globalidentificationofgenesspecificforricemeiosis
AT xumeng globalidentificationofgenesspecificforricemeiosis
AT bianshiquan globalidentificationofgenesspecificforricemeiosis
AT houlili globalidentificationofgenesspecificforricemeiosis
AT tangding globalidentificationofgenesspecificforricemeiosis
AT liyafei globalidentificationofgenesspecificforricemeiosis
AT guminghong globalidentificationofgenesspecificforricemeiosis
AT chengzhukuan globalidentificationofgenesspecificforricemeiosis
AT yuhengxiu globalidentificationofgenesspecificforricemeiosis