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Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes

Little is known about contacts in the spliceosome between proteins and intron nucleotides surrounding the pre-mRNA branch-site and their dynamics during splicing. We investigated protein-pre-mRNA interactions by UV-induced crosslinking of purified yeast B(act) spliceosomes formed on site-specificall...

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Detalles Bibliográficos
Autores principales:	Schneider, Cornelius, Agafonov, Dmitry E., Schmitzová, Jana, Hartmuth, Klaus, Fabrizio, Patrizia, Lührmann, Reinhard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579134/ https://www.ncbi.nlm.nih.gov/pubmed/26393790 http://dx.doi.org/10.1371/journal.pgen.1005539

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