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A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box
Generally, transportation of preimplantation embryos without freezing requires incubators that can maintain an optimal culture environment with a suitable gas phase, temperature, and humidity. Such incubators are expensive to transport. We reported previously that normal offspring were obtained when...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579141/ https://www.ncbi.nlm.nih.gov/pubmed/26393931 http://dx.doi.org/10.1371/journal.pone.0138854 |
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author | Tokoro, Mikiko Fukunaga, Noritaka Yamanaka, Kaori Itoi, Fumiaki Terashita, Yukari Kamada, Yuko Wakayama, Sayaka Asada, Yoshimasa Wakayama, Teruhiko |
author_facet | Tokoro, Mikiko Fukunaga, Noritaka Yamanaka, Kaori Itoi, Fumiaki Terashita, Yukari Kamada, Yuko Wakayama, Sayaka Asada, Yoshimasa Wakayama, Teruhiko |
author_sort | Tokoro, Mikiko |
collection | PubMed |
description | Generally, transportation of preimplantation embryos without freezing requires incubators that can maintain an optimal culture environment with a suitable gas phase, temperature, and humidity. Such incubators are expensive to transport. We reported previously that normal offspring were obtained when the gas phase and temperature could be maintained during transportation. However, that system used plastic dishes for embryo culture and is unsuitable for long-distance transport of live embryos. Here, we developed a simple low-cost embryo transportation system. Instead of plastic dishes, several types of microtubes—usually used for molecular analysis—were tested for embryo culture. When they were washed and attached to a gas-permeable film, the rate of embryo development from the 1-cell to blastocyst stage was more than 90%. The quality of these blastocysts and the rate of full-term development after embryo transfer to recipient female mice were similar to those of a dish-cultured control group. Next, we developed a small warm box powered by a battery instead of mains power, which could maintain an optimal temperature for embryo development during transport. When 1-cell embryos derived from BDF1, C57BL/6, C3H/He and ICR mouse strains were transported by a parcel-delivery service over 3 days using microtubes and the box, they developed to blastocysts with rates similar to controls. After the embryos had been transferred into recipient female mice, healthy offspring were obtained without any losses except for the C3H/He strain. Thus, transport of mouse embryos is possible using this very simple method, which might prove useful in the field of reproductive medicine. |
format | Online Article Text |
id | pubmed-4579141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45791412015-10-01 A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box Tokoro, Mikiko Fukunaga, Noritaka Yamanaka, Kaori Itoi, Fumiaki Terashita, Yukari Kamada, Yuko Wakayama, Sayaka Asada, Yoshimasa Wakayama, Teruhiko PLoS One Research Article Generally, transportation of preimplantation embryos without freezing requires incubators that can maintain an optimal culture environment with a suitable gas phase, temperature, and humidity. Such incubators are expensive to transport. We reported previously that normal offspring were obtained when the gas phase and temperature could be maintained during transportation. However, that system used plastic dishes for embryo culture and is unsuitable for long-distance transport of live embryos. Here, we developed a simple low-cost embryo transportation system. Instead of plastic dishes, several types of microtubes—usually used for molecular analysis—were tested for embryo culture. When they were washed and attached to a gas-permeable film, the rate of embryo development from the 1-cell to blastocyst stage was more than 90%. The quality of these blastocysts and the rate of full-term development after embryo transfer to recipient female mice were similar to those of a dish-cultured control group. Next, we developed a small warm box powered by a battery instead of mains power, which could maintain an optimal temperature for embryo development during transport. When 1-cell embryos derived from BDF1, C57BL/6, C3H/He and ICR mouse strains were transported by a parcel-delivery service over 3 days using microtubes and the box, they developed to blastocysts with rates similar to controls. After the embryos had been transferred into recipient female mice, healthy offspring were obtained without any losses except for the C3H/He strain. Thus, transport of mouse embryos is possible using this very simple method, which might prove useful in the field of reproductive medicine. Public Library of Science 2015-09-22 /pmc/articles/PMC4579141/ /pubmed/26393931 http://dx.doi.org/10.1371/journal.pone.0138854 Text en © 2015 Tokoro et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tokoro, Mikiko Fukunaga, Noritaka Yamanaka, Kaori Itoi, Fumiaki Terashita, Yukari Kamada, Yuko Wakayama, Sayaka Asada, Yoshimasa Wakayama, Teruhiko A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box |
title | A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box |
title_full | A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box |
title_fullStr | A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box |
title_full_unstemmed | A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box |
title_short | A Simple Method for Transportation of Mouse Embryos Using Microtubes and a Warm Box |
title_sort | simple method for transportation of mouse embryos using microtubes and a warm box |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579141/ https://www.ncbi.nlm.nih.gov/pubmed/26393931 http://dx.doi.org/10.1371/journal.pone.0138854 |
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