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Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli

Lycopene is an import ant compound with an increasing industrial value. However, there is still no biotechnological process to obtain it. In this study, a semi-continuous system for lycopene extraction from recombinant Escherichia coli BL21 cells is proposed. A two-phase culture mode using organic s...

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Autores principales: Gallego-Jara, Julia, de Diego, Teresa, del Real, Álvaro, Écija-Conesa, Ana, Manjón, Arturo, Cánovas, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579157/
https://www.ncbi.nlm.nih.gov/pubmed/26395597
http://dx.doi.org/10.1186/s13568-015-0150-3
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author Gallego-Jara, Julia
de Diego, Teresa
del Real, Álvaro
Écija-Conesa, Ana
Manjón, Arturo
Cánovas, Manuel
author_facet Gallego-Jara, Julia
de Diego, Teresa
del Real, Álvaro
Écija-Conesa, Ana
Manjón, Arturo
Cánovas, Manuel
author_sort Gallego-Jara, Julia
collection PubMed
description Lycopene is an import ant compound with an increasing industrial value. However, there is still no biotechnological process to obtain it. In this study, a semi-continuous system for lycopene extraction from recombinant Escherichia coli BL21 cells is proposed. A two-phase culture mode using organic solvents was found to maximize lycopene production through in situ extraction from cells. Within the reactor, three phases were formed during the process: an aqueous phase containing the recombinant E. coli, an interphase, and an organic phase. Lycopene was extracted from the cells to both the interphase and the organic phase and, consequently, thus enhancing its production. Maximum lycopene production (74.71 ± 3.74 mg L(−1)) was obtained for an octane-aqueous culture system using the E. coli BL21LF strain, a process that doubled the level obtained in the control aqueous culture. Study of the interphase by transmission electron microscopy (TEM) showed the proteo-lipidic nature and the high storage capacity of lycopene. Moreover, a cell viability test by flow cytometry (CF) after 24 h of culture indicated that 24 % of the population could be re-used. Therefore, a batch series reactor was designed for semi-continuous lycopene extraction. After five cycles of operation (120 h), lycopene production was similar to that obtained in the control aqueous medium. A final specific lycopene yield of up to 49.70 ± 2.48 mg g(−1) was reached at 24 h, which represents to the highest titer to date. In conclusion, the aqueous-organic semi-continuous culture system proposed is the first designed for lycopene extraction, representing an important breakthrough in the development of a competitive biotechnological process for lycopene production and extraction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-015-0150-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-45791572015-09-25 Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli Gallego-Jara, Julia de Diego, Teresa del Real, Álvaro Écija-Conesa, Ana Manjón, Arturo Cánovas, Manuel AMB Express Original Article Lycopene is an import ant compound with an increasing industrial value. However, there is still no biotechnological process to obtain it. In this study, a semi-continuous system for lycopene extraction from recombinant Escherichia coli BL21 cells is proposed. A two-phase culture mode using organic solvents was found to maximize lycopene production through in situ extraction from cells. Within the reactor, three phases were formed during the process: an aqueous phase containing the recombinant E. coli, an interphase, and an organic phase. Lycopene was extracted from the cells to both the interphase and the organic phase and, consequently, thus enhancing its production. Maximum lycopene production (74.71 ± 3.74 mg L(−1)) was obtained for an octane-aqueous culture system using the E. coli BL21LF strain, a process that doubled the level obtained in the control aqueous culture. Study of the interphase by transmission electron microscopy (TEM) showed the proteo-lipidic nature and the high storage capacity of lycopene. Moreover, a cell viability test by flow cytometry (CF) after 24 h of culture indicated that 24 % of the population could be re-used. Therefore, a batch series reactor was designed for semi-continuous lycopene extraction. After five cycles of operation (120 h), lycopene production was similar to that obtained in the control aqueous medium. A final specific lycopene yield of up to 49.70 ± 2.48 mg g(−1) was reached at 24 h, which represents to the highest titer to date. In conclusion, the aqueous-organic semi-continuous culture system proposed is the first designed for lycopene extraction, representing an important breakthrough in the development of a competitive biotechnological process for lycopene production and extraction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-015-0150-3) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-09-22 /pmc/articles/PMC4579157/ /pubmed/26395597 http://dx.doi.org/10.1186/s13568-015-0150-3 Text en © Gallego-Jara et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Gallego-Jara, Julia
de Diego, Teresa
del Real, Álvaro
Écija-Conesa, Ana
Manjón, Arturo
Cánovas, Manuel
Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli
title Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli
title_full Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli
title_fullStr Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli
title_full_unstemmed Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli
title_short Lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered Escherichia coli
title_sort lycopene overproduction and in situ extraction in organic-aqueous culture systems using a metabolically engineered escherichia coli
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579157/
https://www.ncbi.nlm.nih.gov/pubmed/26395597
http://dx.doi.org/10.1186/s13568-015-0150-3
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