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Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas

BACKGROUND: Changes in the composition of the extracellular matrix (ECM) occur between the proliferating and involuted phases of infantile hemangiomas (IH), and are associated with angiogenic growth. We examined the composition of the ECM in proliferating and involuted IHs and assessed correlations...

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Autores principales: Park, Hyochun, Park, Hannara, Chung, Ho Yun, O, Teresa M, Waner, Milton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Plastic and Reconstructive Surgeons 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579164/
https://www.ncbi.nlm.nih.gov/pubmed/26430624
http://dx.doi.org/10.5999/aps.2015.42.5.544
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author Park, Hyochun
Park, Hannara
Chung, Ho Yun
O, Teresa M
Waner, Milton
author_facet Park, Hyochun
Park, Hannara
Chung, Ho Yun
O, Teresa M
Waner, Milton
author_sort Park, Hyochun
collection PubMed
description BACKGROUND: Changes in the composition of the extracellular matrix (ECM) occur between the proliferating and involuted phases of infantile hemangiomas (IH), and are associated with angiogenic growth. We examined the composition of the ECM in proliferating and involuted IHs and assessed correlations between the composition of the ECM and whether the IH was in the proliferating or the involuted phase. METHODS: We evaluated IH samples from a cohort of patients who had five proliferating IHs and five involuted IHs. The following ECM molecules were analyzed using enzyme-linked immunosorbent assays and immunohistochemistry: laminin, fibronectin, collagen type I, collagen type II, and collagen type III. RESULTS: The involuted IHs had higher levels of deposition of collagen type III than the proliferating IHs. The median values (interquartile ranges) were 1.135 (0.946-1.486) and 1.008 (0.780-1.166) (P=0.019), respectively. The level of laminin was higher in involuted IHs than in proliferating IHs, with median values (interquartile ranges) of 3.191 (2.945-3.191) and 2.479 (1.699-3.284) (P=0.047), respectively. Abundant collagen type III staining was found in involuted IHs. Laminin α4 chain staining was clearly present within the basement membrane adjacent to the blood vessels, and was significantly more intense in involuted IHs than in proliferative IHs. CONCLUSIONS: Involuted hemangiomas showed extensive deposition of collagen III and laminin, suggesting that differences in the composition of the ECM reflect stages of the development of IHs. This pattern may be due to the rapid senescence of IHs.
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spelling pubmed-45791642015-10-01 Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas Park, Hyochun Park, Hannara Chung, Ho Yun O, Teresa M Waner, Milton Arch Plast Surg Original Article BACKGROUND: Changes in the composition of the extracellular matrix (ECM) occur between the proliferating and involuted phases of infantile hemangiomas (IH), and are associated with angiogenic growth. We examined the composition of the ECM in proliferating and involuted IHs and assessed correlations between the composition of the ECM and whether the IH was in the proliferating or the involuted phase. METHODS: We evaluated IH samples from a cohort of patients who had five proliferating IHs and five involuted IHs. The following ECM molecules were analyzed using enzyme-linked immunosorbent assays and immunohistochemistry: laminin, fibronectin, collagen type I, collagen type II, and collagen type III. RESULTS: The involuted IHs had higher levels of deposition of collagen type III than the proliferating IHs. The median values (interquartile ranges) were 1.135 (0.946-1.486) and 1.008 (0.780-1.166) (P=0.019), respectively. The level of laminin was higher in involuted IHs than in proliferating IHs, with median values (interquartile ranges) of 3.191 (2.945-3.191) and 2.479 (1.699-3.284) (P=0.047), respectively. Abundant collagen type III staining was found in involuted IHs. Laminin α4 chain staining was clearly present within the basement membrane adjacent to the blood vessels, and was significantly more intense in involuted IHs than in proliferative IHs. CONCLUSIONS: Involuted hemangiomas showed extensive deposition of collagen III and laminin, suggesting that differences in the composition of the ECM reflect stages of the development of IHs. This pattern may be due to the rapid senescence of IHs. The Korean Society of Plastic and Reconstructive Surgeons 2015-09 2015-09-15 /pmc/articles/PMC4579164/ /pubmed/26430624 http://dx.doi.org/10.5999/aps.2015.42.5.544 Text en Copyright © 2015 The Korean Society of Plastic and Reconstructive Surgeons http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Hyochun
Park, Hannara
Chung, Ho Yun
O, Teresa M
Waner, Milton
Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas
title Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas
title_full Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas
title_fullStr Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas
title_full_unstemmed Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas
title_short Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas
title_sort comparative analysis of the extracellular matrix composition in proliferating and involuted infantile hemangiomas
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579164/
https://www.ncbi.nlm.nih.gov/pubmed/26430624
http://dx.doi.org/10.5999/aps.2015.42.5.544
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