Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs

BACKGROUND: The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele. RESULTS: A quantitative PCR test was de...

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Autores principales: Waldo, Jennifer Turner, Diaz, Kasandra Santana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579391/
https://www.ncbi.nlm.nih.gov/pubmed/26401330
http://dx.doi.org/10.1186/s40575-015-0013-x
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author Waldo, Jennifer Turner
Diaz, Kasandra Santana
author_facet Waldo, Jennifer Turner
Diaz, Kasandra Santana
author_sort Waldo, Jennifer Turner
collection PubMed
description BACKGROUND: The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele. RESULTS: A quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally. CONCLUSIONS: This work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele.
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spelling pubmed-45793912015-09-23 Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs Waldo, Jennifer Turner Diaz, Kasandra Santana Canine Genet Epidemiol Research BACKGROUND: The breed-defining dorsal ridge in Rhodesian Ridgeback dogs is the result of a 133,000 base pair duplication on chromosome 18. Because this trait is dominant, heterozygous dogs cannot be discriminated from those with two copies of the Ridge allele. RESULTS: A quantitative PCR test was developed and dogs of known genotype were used as test subjects. In all cases, the correct genotype was determined experimentally. CONCLUSIONS: This work provides a rapid and accurate methodology for determining dog genotype with respect to the Ridge allele. BioMed Central 2015-01-27 /pmc/articles/PMC4579391/ /pubmed/26401330 http://dx.doi.org/10.1186/s40575-015-0013-x Text en © Waldo and Diaz; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Waldo, Jennifer Turner
Diaz, Kasandra Santana
Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs
title Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs
title_full Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs
title_fullStr Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs
title_full_unstemmed Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs
title_short Development and validation of a diagnostic test for Ridge allele copy number in Rhodesian Ridgeback dogs
title_sort development and validation of a diagnostic test for ridge allele copy number in rhodesian ridgeback dogs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579391/
https://www.ncbi.nlm.nih.gov/pubmed/26401330
http://dx.doi.org/10.1186/s40575-015-0013-x
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