Cargando…

Riverbed methanotrophy sustained by high carbon conversion efficiency

Our understanding of the role of freshwaters in the global carbon cycle is being revised, but there is still a lack of data, especially for the cycling of methane, in rivers and streams. Unravelling the role of methanotrophy is key to determining the fate of methane in rivers. Here we focus on the c...

Descripción completa

Detalles Bibliográficos
Autores principales: Trimmer, Mark, Shelley, Felicity C, Purdy, Kevin J, Maanoja, Susanna T, Chronopoulou, Panagiota-Myrsini, Grey, Jonathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579481/
https://www.ncbi.nlm.nih.gov/pubmed/26057842
http://dx.doi.org/10.1038/ismej.2015.98
_version_ 1782391265619345408
author Trimmer, Mark
Shelley, Felicity C
Purdy, Kevin J
Maanoja, Susanna T
Chronopoulou, Panagiota-Myrsini
Grey, Jonathan
author_facet Trimmer, Mark
Shelley, Felicity C
Purdy, Kevin J
Maanoja, Susanna T
Chronopoulou, Panagiota-Myrsini
Grey, Jonathan
author_sort Trimmer, Mark
collection PubMed
description Our understanding of the role of freshwaters in the global carbon cycle is being revised, but there is still a lack of data, especially for the cycling of methane, in rivers and streams. Unravelling the role of methanotrophy is key to determining the fate of methane in rivers. Here we focus on the carbon conversion efficiency (CCE) of methanotrophy, that is, how much organic carbon is produced per mole of CH(4) oxidised, and how this is influenced by variation in methanotroph communities. First, we show that the CCE of riverbed methanotrophs is consistently high (~50%) across a wide range of methane concentrations (~10–7000 nM) and despite a 10-fold span in the rate of methane oxidation. Then, we show that this high conversion efficiency is largely conserved (50%± confidence interval 44–56%) across pronounced variation in the key functional gene (70 operational taxonomic units (OTUs)), particulate methane monooxygenase (pmoA), and marked shifts in the abundance of Type I and Type II methanotrophs in eight replicate chalk streams. These data may suggest a degree of functional redundancy within the variable methanotroph community inhabiting these streams and that some of the variation in pmoA may reflect a suite of enzymes of different methane affinities which enables such a large range of methane concentrations to be oxidised. The latter, coupled to their high CCE, enables the methanotrophs to sustain net production throughout the year, regardless of the marked temporal and spatial changes that occur in methane.
format Online
Article
Text
id pubmed-4579481
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-45794812015-10-01 Riverbed methanotrophy sustained by high carbon conversion efficiency Trimmer, Mark Shelley, Felicity C Purdy, Kevin J Maanoja, Susanna T Chronopoulou, Panagiota-Myrsini Grey, Jonathan ISME J Original Article Our understanding of the role of freshwaters in the global carbon cycle is being revised, but there is still a lack of data, especially for the cycling of methane, in rivers and streams. Unravelling the role of methanotrophy is key to determining the fate of methane in rivers. Here we focus on the carbon conversion efficiency (CCE) of methanotrophy, that is, how much organic carbon is produced per mole of CH(4) oxidised, and how this is influenced by variation in methanotroph communities. First, we show that the CCE of riverbed methanotrophs is consistently high (~50%) across a wide range of methane concentrations (~10–7000 nM) and despite a 10-fold span in the rate of methane oxidation. Then, we show that this high conversion efficiency is largely conserved (50%± confidence interval 44–56%) across pronounced variation in the key functional gene (70 operational taxonomic units (OTUs)), particulate methane monooxygenase (pmoA), and marked shifts in the abundance of Type I and Type II methanotrophs in eight replicate chalk streams. These data may suggest a degree of functional redundancy within the variable methanotroph community inhabiting these streams and that some of the variation in pmoA may reflect a suite of enzymes of different methane affinities which enables such a large range of methane concentrations to be oxidised. The latter, coupled to their high CCE, enables the methanotrophs to sustain net production throughout the year, regardless of the marked temporal and spatial changes that occur in methane. Nature Publishing Group 2015-10 2015-06-09 /pmc/articles/PMC4579481/ /pubmed/26057842 http://dx.doi.org/10.1038/ismej.2015.98 Text en Copyright © 2015 International Society for Microbial Ecology http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Original Article
Trimmer, Mark
Shelley, Felicity C
Purdy, Kevin J
Maanoja, Susanna T
Chronopoulou, Panagiota-Myrsini
Grey, Jonathan
Riverbed methanotrophy sustained by high carbon conversion efficiency
title Riverbed methanotrophy sustained by high carbon conversion efficiency
title_full Riverbed methanotrophy sustained by high carbon conversion efficiency
title_fullStr Riverbed methanotrophy sustained by high carbon conversion efficiency
title_full_unstemmed Riverbed methanotrophy sustained by high carbon conversion efficiency
title_short Riverbed methanotrophy sustained by high carbon conversion efficiency
title_sort riverbed methanotrophy sustained by high carbon conversion efficiency
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579481/
https://www.ncbi.nlm.nih.gov/pubmed/26057842
http://dx.doi.org/10.1038/ismej.2015.98
work_keys_str_mv AT trimmermark riverbedmethanotrophysustainedbyhighcarbonconversionefficiency
AT shelleyfelicityc riverbedmethanotrophysustainedbyhighcarbonconversionefficiency
AT purdykevinj riverbedmethanotrophysustainedbyhighcarbonconversionefficiency
AT maanojasusannat riverbedmethanotrophysustainedbyhighcarbonconversionefficiency
AT chronopouloupanagiotamyrsini riverbedmethanotrophysustainedbyhighcarbonconversionefficiency
AT greyjonathan riverbedmethanotrophysustainedbyhighcarbonconversionefficiency