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Nuclear envelope-associated endosomes deliver surface proteins to the nucleus
Endocytosis directs molecular cargo along three main routes: recycling to the cell surface, transport to the Golgi apparatus or degradation in endolysosomes. Pseudomonas exotoxin A (PE) is a bacterial protein that typically traffics to the Golgi and then the endoplasmic reticulum before translocatin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579783/ https://www.ncbi.nlm.nih.gov/pubmed/26356418 http://dx.doi.org/10.1038/ncomms9218 |
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author | Chaumet, Alexandre Wright, Graham D. Seet, Sze Hwee Tham, Keit Min Gounko, Natalia V. Bard, Frederic |
author_facet | Chaumet, Alexandre Wright, Graham D. Seet, Sze Hwee Tham, Keit Min Gounko, Natalia V. Bard, Frederic |
author_sort | Chaumet, Alexandre |
collection | PubMed |
description | Endocytosis directs molecular cargo along three main routes: recycling to the cell surface, transport to the Golgi apparatus or degradation in endolysosomes. Pseudomonas exotoxin A (PE) is a bacterial protein that typically traffics to the Golgi and then the endoplasmic reticulum before translocating to the cytosol. Here we show that a substantial fraction of internalized PE is also located in nuclear envelope-associated endosomes (NAE), which display limited mobility, exhibit a propensity to undergo fusion and readily discharge their contents into the nuclear envelope. Electron microscopy and protein trapping in the nucleus indicate that NAE mediate PE transfer into the nucleoplasm. RNAi screening further revealed that NAE-mediated transfer depends on the nuclear envelope proteins SUN1 and SUN2, as well as the Sec61 translocon complex. These data reveal a novel endosomal route from the cell surface to the nucleoplasm that facilitates the accumulation of extracellular and cell surface proteins in the nucleus. |
format | Online Article Text |
id | pubmed-4579783 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45797832015-10-01 Nuclear envelope-associated endosomes deliver surface proteins to the nucleus Chaumet, Alexandre Wright, Graham D. Seet, Sze Hwee Tham, Keit Min Gounko, Natalia V. Bard, Frederic Nat Commun Article Endocytosis directs molecular cargo along three main routes: recycling to the cell surface, transport to the Golgi apparatus or degradation in endolysosomes. Pseudomonas exotoxin A (PE) is a bacterial protein that typically traffics to the Golgi and then the endoplasmic reticulum before translocating to the cytosol. Here we show that a substantial fraction of internalized PE is also located in nuclear envelope-associated endosomes (NAE), which display limited mobility, exhibit a propensity to undergo fusion and readily discharge their contents into the nuclear envelope. Electron microscopy and protein trapping in the nucleus indicate that NAE mediate PE transfer into the nucleoplasm. RNAi screening further revealed that NAE-mediated transfer depends on the nuclear envelope proteins SUN1 and SUN2, as well as the Sec61 translocon complex. These data reveal a novel endosomal route from the cell surface to the nucleoplasm that facilitates the accumulation of extracellular and cell surface proteins in the nucleus. Nature Pub. Group 2015-09-10 /pmc/articles/PMC4579783/ /pubmed/26356418 http://dx.doi.org/10.1038/ncomms9218 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chaumet, Alexandre Wright, Graham D. Seet, Sze Hwee Tham, Keit Min Gounko, Natalia V. Bard, Frederic Nuclear envelope-associated endosomes deliver surface proteins to the nucleus |
title | Nuclear envelope-associated endosomes deliver surface proteins to the nucleus |
title_full | Nuclear envelope-associated endosomes deliver surface proteins to the nucleus |
title_fullStr | Nuclear envelope-associated endosomes deliver surface proteins to the nucleus |
title_full_unstemmed | Nuclear envelope-associated endosomes deliver surface proteins to the nucleus |
title_short | Nuclear envelope-associated endosomes deliver surface proteins to the nucleus |
title_sort | nuclear envelope-associated endosomes deliver surface proteins to the nucleus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579783/ https://www.ncbi.nlm.nih.gov/pubmed/26356418 http://dx.doi.org/10.1038/ncomms9218 |
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