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High expression of the putative cancer stem cell marker, DCLK1, in rectal neuroendocrine tumors

Doublecortin-like kinase 1 (DCLK1), a microtubule-associated protein, is known to regulate neuronal differentiation, migration and neurogenesis. Recent evidence suggests that the protein is a putative marker for intestinal and pancreatic stem cells, including their cancer stem cell counterparts. The...

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Autores principales: IKEZONO, YU, KOGA, HIRONORI, ABE, MITSUHIKO, AKIBA, JUN, KAWAHARA, AKIHIKO, YOSHIDA, TAKAFUMI, NAKAMURA, TORU, IWAMOTO, HIDEKI, YANO, HIROHISA, KAGE, MASAYOSHI, SATA, MICHIO, TSURUTA, OSAMU, TORIMURA, TAKUJI
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579808/
https://www.ncbi.nlm.nih.gov/pubmed/26622789
http://dx.doi.org/10.3892/ol.2015.3513
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author IKEZONO, YU
KOGA, HIRONORI
ABE, MITSUHIKO
AKIBA, JUN
KAWAHARA, AKIHIKO
YOSHIDA, TAKAFUMI
NAKAMURA, TORU
IWAMOTO, HIDEKI
YANO, HIROHISA
KAGE, MASAYOSHI
SATA, MICHIO
TSURUTA, OSAMU
TORIMURA, TAKUJI
author_facet IKEZONO, YU
KOGA, HIRONORI
ABE, MITSUHIKO
AKIBA, JUN
KAWAHARA, AKIHIKO
YOSHIDA, TAKAFUMI
NAKAMURA, TORU
IWAMOTO, HIDEKI
YANO, HIROHISA
KAGE, MASAYOSHI
SATA, MICHIO
TSURUTA, OSAMU
TORIMURA, TAKUJI
author_sort IKEZONO, YU
collection PubMed
description Doublecortin-like kinase 1 (DCLK1), a microtubule-associated protein, is known to regulate neuronal differentiation, migration and neurogenesis. Recent evidence suggests that the protein is a putative marker for intestinal and pancreatic stem cells, including their cancer stem cell counterparts. The present study conducted immunohistochemical analyses for DCLK1 and the stemness marker, NANOG, in human intestinal neuroendocrine tumors (NETs), as their expression had not been previously investigated in these tumors. Eighteen patients with endoscopically resected rectal NETs were enrolled in the study. The mean age of the patients was 51 years old. The mean diameter of the resected tumors was 5.2 mm, and a histological diagnosis of NET grade G1 was formed for all tumors. Immunohistochemical analysis was performed not only for DCLK1, but also for the known NET markers, synaptophysin, chromogranin A and cluster of differentiation (CD)56. The intensity and distribution of staining were scored on a scale of 0–3 and 0–2, respectively. The sum of the scores was calculated for each specimen. Co-expression of DCLK1 and NANOG was also examined. The mean scores for DCLK1 and synaptophysin were significantly higher than those for chromogranin A (P<0.0001) and CD56 (P<0.01). There were no significant differences in the scores between DCLK1 and synaptophysin or between chromogranin A and CD56. Notably, NANOG was expressed in high quantities in all the tumor tissues studied, showing clear co-expression with DCLK1. In conclusion, DCLK1 may be a novel marker for rectal NET, potentially indicating the presence of the stemness gene product, NANOG.
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spelling pubmed-45798082015-11-30 High expression of the putative cancer stem cell marker, DCLK1, in rectal neuroendocrine tumors IKEZONO, YU KOGA, HIRONORI ABE, MITSUHIKO AKIBA, JUN KAWAHARA, AKIHIKO YOSHIDA, TAKAFUMI NAKAMURA, TORU IWAMOTO, HIDEKI YANO, HIROHISA KAGE, MASAYOSHI SATA, MICHIO TSURUTA, OSAMU TORIMURA, TAKUJI Oncol Lett Articles Doublecortin-like kinase 1 (DCLK1), a microtubule-associated protein, is known to regulate neuronal differentiation, migration and neurogenesis. Recent evidence suggests that the protein is a putative marker for intestinal and pancreatic stem cells, including their cancer stem cell counterparts. The present study conducted immunohistochemical analyses for DCLK1 and the stemness marker, NANOG, in human intestinal neuroendocrine tumors (NETs), as their expression had not been previously investigated in these tumors. Eighteen patients with endoscopically resected rectal NETs were enrolled in the study. The mean age of the patients was 51 years old. The mean diameter of the resected tumors was 5.2 mm, and a histological diagnosis of NET grade G1 was formed for all tumors. Immunohistochemical analysis was performed not only for DCLK1, but also for the known NET markers, synaptophysin, chromogranin A and cluster of differentiation (CD)56. The intensity and distribution of staining were scored on a scale of 0–3 and 0–2, respectively. The sum of the scores was calculated for each specimen. Co-expression of DCLK1 and NANOG was also examined. The mean scores for DCLK1 and synaptophysin were significantly higher than those for chromogranin A (P<0.0001) and CD56 (P<0.01). There were no significant differences in the scores between DCLK1 and synaptophysin or between chromogranin A and CD56. Notably, NANOG was expressed in high quantities in all the tumor tissues studied, showing clear co-expression with DCLK1. In conclusion, DCLK1 may be a novel marker for rectal NET, potentially indicating the presence of the stemness gene product, NANOG. D.A. Spandidos 2015-10 2015-07-20 /pmc/articles/PMC4579808/ /pubmed/26622789 http://dx.doi.org/10.3892/ol.2015.3513 Text en Copyright: © Ikezono et al. This is an open access article distributed under the terms of a Creative Commons Attribution License. http://creativecommons.org/licenses/by/4.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
IKEZONO, YU
KOGA, HIRONORI
ABE, MITSUHIKO
AKIBA, JUN
KAWAHARA, AKIHIKO
YOSHIDA, TAKAFUMI
NAKAMURA, TORU
IWAMOTO, HIDEKI
YANO, HIROHISA
KAGE, MASAYOSHI
SATA, MICHIO
TSURUTA, OSAMU
TORIMURA, TAKUJI
High expression of the putative cancer stem cell marker, DCLK1, in rectal neuroendocrine tumors
title High expression of the putative cancer stem cell marker, DCLK1, in rectal neuroendocrine tumors
title_full High expression of the putative cancer stem cell marker, DCLK1, in rectal neuroendocrine tumors
title_fullStr High expression of the putative cancer stem cell marker, DCLK1, in rectal neuroendocrine tumors
title_full_unstemmed High expression of the putative cancer stem cell marker, DCLK1, in rectal neuroendocrine tumors
title_short High expression of the putative cancer stem cell marker, DCLK1, in rectal neuroendocrine tumors
title_sort high expression of the putative cancer stem cell marker, dclk1, in rectal neuroendocrine tumors
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579808/
https://www.ncbi.nlm.nih.gov/pubmed/26622789
http://dx.doi.org/10.3892/ol.2015.3513
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