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Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice

Although high density lipoprotein (HDL) improves the functions of endothelial progenitor cells (EPCs), the effect of HDL ApoAI mimetic peptide reverse-D-4F (Rev-D4F) on EPC mobilization and repair of EPC dysfunctions remains to be studied. In this study, we investigated the effects of Rev-D4F on per...

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Autores principales: Nana, Yang, Peng, Jiao, Jianlin, Zhang, Xiangjian, Zhang, Shutong, Yao, Enxin, Zhan, Bin, Li, Chuanlong, Zong, Hua, Tian, Yanhong, Si, Yunsai, Du, Shucun, Qin, Hui, Wang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580448/
https://www.ncbi.nlm.nih.gov/pubmed/26398523
http://dx.doi.org/10.1371/journal.pone.0138832
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author Nana, Yang
Peng, Jiao
Jianlin, Zhang
Xiangjian, Zhang
Shutong, Yao
Enxin, Zhan
Bin, Li
Chuanlong, Zong
Hua, Tian
Yanhong, Si
Yunsai, Du
Shucun, Qin
Hui, Wang
author_facet Nana, Yang
Peng, Jiao
Jianlin, Zhang
Xiangjian, Zhang
Shutong, Yao
Enxin, Zhan
Bin, Li
Chuanlong, Zong
Hua, Tian
Yanhong, Si
Yunsai, Du
Shucun, Qin
Hui, Wang
author_sort Nana, Yang
collection PubMed
description Although high density lipoprotein (HDL) improves the functions of endothelial progenitor cells (EPCs), the effect of HDL ApoAI mimetic peptide reverse-D-4F (Rev-D4F) on EPC mobilization and repair of EPC dysfunctions remains to be studied. In this study, we investigated the effects of Rev-D4F on peripheral blood cell subpopulations in C57 mice treated with a high fat diet and the mechanism of Rev-D4F in improving the function of EPCs impaired by tumor necrosis factor-α (TNF-α). The high fat diet significantly decreased the number of EPCs, EPC migratory functions, and the percentage of lymphocytes in the white blood cells. However, it significantly increased the number of white blood cells, the percentage of monocytes in the white blood cells, and the level of vascular endothelial growth factor (VEGF) and TNF-α in the plasma. Rev-D4F clearly inhibited the effect of the high fat diet on the quantification of peripheral blood cell subpopulations and cytokine levels, and increased stromal cell derived factor 1α (SDF-1α) in the plasma. We provided in vitro evidence that TNF-α impaired EPC proliferation, migration, and tube formation through inactive AKT and eNOS, which was restored by Rev-D4F treatment. In contrast, both the PI3-kinase (PI3K) inhibitor (LY294002) and AKT inhibitor (perifosine) obviously inhibited the restoration of Rev-4F on EPCs impaired by TNF-α. Our results suggested that Rev-D4F increases the quantity of endothelial progenitor cells through increasing the SDF-1α levels and decreasing the TNF-α level of peripheral blood in high fat diet-induced C57BL/6J mice, and restores TNF-α induced dysfunctions of EPCs partly through stimulating the PI3K/AKT signal pathway.
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spelling pubmed-45804482015-10-01 Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice Nana, Yang Peng, Jiao Jianlin, Zhang Xiangjian, Zhang Shutong, Yao Enxin, Zhan Bin, Li Chuanlong, Zong Hua, Tian Yanhong, Si Yunsai, Du Shucun, Qin Hui, Wang PLoS One Research Article Although high density lipoprotein (HDL) improves the functions of endothelial progenitor cells (EPCs), the effect of HDL ApoAI mimetic peptide reverse-D-4F (Rev-D4F) on EPC mobilization and repair of EPC dysfunctions remains to be studied. In this study, we investigated the effects of Rev-D4F on peripheral blood cell subpopulations in C57 mice treated with a high fat diet and the mechanism of Rev-D4F in improving the function of EPCs impaired by tumor necrosis factor-α (TNF-α). The high fat diet significantly decreased the number of EPCs, EPC migratory functions, and the percentage of lymphocytes in the white blood cells. However, it significantly increased the number of white blood cells, the percentage of monocytes in the white blood cells, and the level of vascular endothelial growth factor (VEGF) and TNF-α in the plasma. Rev-D4F clearly inhibited the effect of the high fat diet on the quantification of peripheral blood cell subpopulations and cytokine levels, and increased stromal cell derived factor 1α (SDF-1α) in the plasma. We provided in vitro evidence that TNF-α impaired EPC proliferation, migration, and tube formation through inactive AKT and eNOS, which was restored by Rev-D4F treatment. In contrast, both the PI3-kinase (PI3K) inhibitor (LY294002) and AKT inhibitor (perifosine) obviously inhibited the restoration of Rev-4F on EPCs impaired by TNF-α. Our results suggested that Rev-D4F increases the quantity of endothelial progenitor cells through increasing the SDF-1α levels and decreasing the TNF-α level of peripheral blood in high fat diet-induced C57BL/6J mice, and restores TNF-α induced dysfunctions of EPCs partly through stimulating the PI3K/AKT signal pathway. Public Library of Science 2015-09-23 /pmc/articles/PMC4580448/ /pubmed/26398523 http://dx.doi.org/10.1371/journal.pone.0138832 Text en © 2015 Nana et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nana, Yang
Peng, Jiao
Jianlin, Zhang
Xiangjian, Zhang
Shutong, Yao
Enxin, Zhan
Bin, Li
Chuanlong, Zong
Hua, Tian
Yanhong, Si
Yunsai, Du
Shucun, Qin
Hui, Wang
Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice
title Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice
title_full Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice
title_fullStr Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice
title_full_unstemmed Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice
title_short Reverse-D-4F Increases the Number of Endothelial Progenitor Cells and Improves Endothelial Progenitor Cell Dysfunctions in High Fat Diet Mice
title_sort reverse-d-4f increases the number of endothelial progenitor cells and improves endothelial progenitor cell dysfunctions in high fat diet mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580448/
https://www.ncbi.nlm.nih.gov/pubmed/26398523
http://dx.doi.org/10.1371/journal.pone.0138832
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