Cargando…

Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells

Since the first mammal was cloned, the idea of using this technique to help endangered species has aroused considerable interest. However, several issues limit this possibility, including the relatively low success rate at every stage of the cloning process, and the dearth of usable tissues from the...

Descripción completa

Detalles Bibliográficos
Autores principales: Prescott, Hilary M. A., Manning, Craig, Gardner, Aaron, Ritchie, William A., Pizzi, Romain, Girling, Simon, Valentine, Iain, Wang, Chengdong, Jahoda, Colin A. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580591/
https://www.ncbi.nlm.nih.gov/pubmed/26398672
http://dx.doi.org/10.1371/journal.pone.0138840
_version_ 1782391415100145664
author Prescott, Hilary M. A.
Manning, Craig
Gardner, Aaron
Ritchie, William A.
Pizzi, Romain
Girling, Simon
Valentine, Iain
Wang, Chengdong
Jahoda, Colin A. B.
author_facet Prescott, Hilary M. A.
Manning, Craig
Gardner, Aaron
Ritchie, William A.
Pizzi, Romain
Girling, Simon
Valentine, Iain
Wang, Chengdong
Jahoda, Colin A. B.
author_sort Prescott, Hilary M. A.
collection PubMed
description Since the first mammal was cloned, the idea of using this technique to help endangered species has aroused considerable interest. However, several issues limit this possibility, including the relatively low success rate at every stage of the cloning process, and the dearth of usable tissues from these rare animals. iPS cells have been produced from cells from a number of rare mammalian species and this is the method of choice for strategies to improve cloning efficiency and create new gametes by directed differentiation. Nevertheless information about other stem cell/progenitor capabilities of cells from endangered species could prove important for future conservation approaches and adds to the knowledge base about cellular material that can be extremely limited. Multipotent progenitor cells, termed skin-derived precursor (SKP) cells, can be isolated directly from mammalian skin dermis, and human cheek tissue has also been shown to be a good source of SKP-like cells. Recently we showed that structures identical to SKPs termed m-SKPs could be obtained from monolayer/ two dimensional (2D) skin fibroblast cultures. Here we aimed to isolate m-SKPs from cultured cells of three endangered species; giant panda (Ailuropoda melanoleuca); red panda (Ailurus fulgens); and Asiatic lion (Panthera leo persica). m-SKP-like spheres were formed from the giant panda buccal mucosa fibroblasts; whereas dermal fibroblast (DF) cells cultured from abdominal skin of the other two species were unable to generate spheres. Under specific differentiation culture conditions giant panda spheres expressed neural, Schwann, adipogenic and osteogenic cell markers. Furthermore, these buccal mucosa derived spheres were shown to maintain expression of SKP markers: nestin, versican, fibronectin, and P75 and switch on expression of the stem cell marker ABCG2. These results demonstrate that giant panda cheek skin can be a useful source of m-SKP multipotent progenitors. At present lack of sample numbers means that we can only postulate why we were unable to obtain m-SKPs from the lion and red panda cultures. However the giant panda observations point to the value of archiving cells from rare species, and the possibilities for later progenitor cell derivation.
format Online
Article
Text
id pubmed-4580591
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45805912015-10-01 Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells Prescott, Hilary M. A. Manning, Craig Gardner, Aaron Ritchie, William A. Pizzi, Romain Girling, Simon Valentine, Iain Wang, Chengdong Jahoda, Colin A. B. PLoS One Research Article Since the first mammal was cloned, the idea of using this technique to help endangered species has aroused considerable interest. However, several issues limit this possibility, including the relatively low success rate at every stage of the cloning process, and the dearth of usable tissues from these rare animals. iPS cells have been produced from cells from a number of rare mammalian species and this is the method of choice for strategies to improve cloning efficiency and create new gametes by directed differentiation. Nevertheless information about other stem cell/progenitor capabilities of cells from endangered species could prove important for future conservation approaches and adds to the knowledge base about cellular material that can be extremely limited. Multipotent progenitor cells, termed skin-derived precursor (SKP) cells, can be isolated directly from mammalian skin dermis, and human cheek tissue has also been shown to be a good source of SKP-like cells. Recently we showed that structures identical to SKPs termed m-SKPs could be obtained from monolayer/ two dimensional (2D) skin fibroblast cultures. Here we aimed to isolate m-SKPs from cultured cells of three endangered species; giant panda (Ailuropoda melanoleuca); red panda (Ailurus fulgens); and Asiatic lion (Panthera leo persica). m-SKP-like spheres were formed from the giant panda buccal mucosa fibroblasts; whereas dermal fibroblast (DF) cells cultured from abdominal skin of the other two species were unable to generate spheres. Under specific differentiation culture conditions giant panda spheres expressed neural, Schwann, adipogenic and osteogenic cell markers. Furthermore, these buccal mucosa derived spheres were shown to maintain expression of SKP markers: nestin, versican, fibronectin, and P75 and switch on expression of the stem cell marker ABCG2. These results demonstrate that giant panda cheek skin can be a useful source of m-SKP multipotent progenitors. At present lack of sample numbers means that we can only postulate why we were unable to obtain m-SKPs from the lion and red panda cultures. However the giant panda observations point to the value of archiving cells from rare species, and the possibilities for later progenitor cell derivation. Public Library of Science 2015-09-23 /pmc/articles/PMC4580591/ /pubmed/26398672 http://dx.doi.org/10.1371/journal.pone.0138840 Text en © 2015 Prescott et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Prescott, Hilary M. A.
Manning, Craig
Gardner, Aaron
Ritchie, William A.
Pizzi, Romain
Girling, Simon
Valentine, Iain
Wang, Chengdong
Jahoda, Colin A. B.
Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells
title Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells
title_full Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells
title_fullStr Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells
title_full_unstemmed Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells
title_short Giant Panda (Ailuropoda melanoleuca) Buccal Mucosa Tissue as a Source of Multipotent Progenitor Cells
title_sort giant panda (ailuropoda melanoleuca) buccal mucosa tissue as a source of multipotent progenitor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580591/
https://www.ncbi.nlm.nih.gov/pubmed/26398672
http://dx.doi.org/10.1371/journal.pone.0138840
work_keys_str_mv AT prescotthilaryma giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells
AT manningcraig giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells
AT gardneraaron giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells
AT ritchiewilliama giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells
AT pizziromain giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells
AT girlingsimon giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells
AT valentineiain giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells
AT wangchengdong giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells
AT jahodacolinab giantpandaailuropodamelanoleucabuccalmucosatissueasasourceofmultipotentprogenitorcells