Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology

This study investigated the comparative accuracy of a recombinant 56-kDa type-specific antigen-based rapid diagnostic test (RDT) for scrub typhus for the detection of IgM antibodies by using conventional serology in well-characterized serum samples from undifferentiated febrile illness patients. The...

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Autores principales: Kingston, Hugh W. F., Blacksell, Stuart D., Tanganuchitcharnchai, Ampai, Laongnualpanich, Achara, Basnyat, Buddha, Day, Nicholas P. J., Paris, Daniel H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2015
Materias:
Diagnostic Laboratory Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580738/
https://www.ncbi.nlm.nih.gov/pubmed/26291089
http://dx.doi.org/10.1128/CVI.00390-15
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author Kingston, Hugh W. F.
Blacksell, Stuart D.
Tanganuchitcharnchai, Ampai
Laongnualpanich, Achara
Basnyat, Buddha
Day, Nicholas P. J.
Paris, Daniel H.
author_facet Kingston, Hugh W. F.
Blacksell, Stuart D.
Tanganuchitcharnchai, Ampai
Laongnualpanich, Achara
Basnyat, Buddha
Day, Nicholas P. J.
Paris, Daniel H.
author_sort Kingston, Hugh W. F.
collection PubMed
description This study investigated the comparative accuracy of a recombinant 56-kDa type-specific antigen-based rapid diagnostic test (RDT) for scrub typhus for the detection of IgM antibodies by using conventional serology in well-characterized serum samples from undifferentiated febrile illness patients. The RDT showed high specificity and promising comparative accuracy, with 82% sensitivity and 98% specificity for samples defined positive at an IgM indirect immunofluorescence assay positivity cutoff titer of ≥1:1,600 versus 92% and 95% at ≥1:6,400, respectively.
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spelling pubmed-45807382015-10-25 Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology Kingston, Hugh W. F. Blacksell, Stuart D. Tanganuchitcharnchai, Ampai Laongnualpanich, Achara Basnyat, Buddha Day, Nicholas P. J. Paris, Daniel H. Clin Vaccine Immunol Diagnostic Laboratory Immunology This study investigated the comparative accuracy of a recombinant 56-kDa type-specific antigen-based rapid diagnostic test (RDT) for scrub typhus for the detection of IgM antibodies by using conventional serology in well-characterized serum samples from undifferentiated febrile illness patients. The RDT showed high specificity and promising comparative accuracy, with 82% sensitivity and 98% specificity for samples defined positive at an IgM indirect immunofluorescence assay positivity cutoff titer of ≥1:1,600 versus 92% and 95% at ≥1:6,400, respectively. American Society for Microbiology 2015-09-23 2015-10 /pmc/articles/PMC4580738/ /pubmed/26291089 http://dx.doi.org/10.1128/CVI.00390-15 Text en Copyright © 2015, Kingston et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Diagnostic Laboratory Immunology
Kingston, Hugh W. F.
Blacksell, Stuart D.
Tanganuchitcharnchai, Ampai
Laongnualpanich, Achara
Basnyat, Buddha
Day, Nicholas P. J.
Paris, Daniel H.
Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology
title Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology
title_full Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology
title_fullStr Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology
title_full_unstemmed Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology
title_short Comparative Accuracy of the InBios Scrub Typhus Detect IgM Rapid Test for the Detection of IgM Antibodies by Using Conventional Serology
title_sort comparative accuracy of the inbios scrub typhus detect igm rapid test for the detection of igm antibodies by using conventional serology
topic Diagnostic Laboratory Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580738/
https://www.ncbi.nlm.nih.gov/pubmed/26291089
http://dx.doi.org/10.1128/CVI.00390-15
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