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Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons

Cells undergo apoptosis mainly via two pathways-the mitochondrial pathway and the cytosolic pathway. It has been well documented that activation of the mitochondrial pathway promotes mitochondrial fragmentation and inhibition of mitochondrial fragmentation partly represses cell death. However, the m...

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Autores principales: Cho, Hyo Min, Sun, Woong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Brain and Neural Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580749/
https://www.ncbi.nlm.nih.gov/pubmed/26412971
http://dx.doi.org/10.5607/en.2015.24.3.219
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author Cho, Hyo Min
Sun, Woong
author_facet Cho, Hyo Min
Sun, Woong
author_sort Cho, Hyo Min
collection PubMed
description Cells undergo apoptosis mainly via two pathways-the mitochondrial pathway and the cytosolic pathway. It has been well documented that activation of the mitochondrial pathway promotes mitochondrial fragmentation and inhibition of mitochondrial fragmentation partly represses cell death. However, the mitochondrial events following activation of the cytosolic pathway are less understood. In this study, we treated Fas-activating antibody and found mitochondrial fragmentation without cell death in hippocampal primary neurons and HT-22 cell lines. Fas antibody treatment, in fact, promoted rapid activation of caspase-8, while executioner caspase-3 activation was not observed. Furthermore, blockage of caspase-8 efficiently prevented Fas antibody-induced mitochondrial fragmentation. These results suggest that the cytosolic pathway induced by death receptor activation promotes caspase-8-dependent mitochondrial fission.
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spelling pubmed-45807492015-09-25 Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons Cho, Hyo Min Sun, Woong Exp Neurobiol Original Article Cells undergo apoptosis mainly via two pathways-the mitochondrial pathway and the cytosolic pathway. It has been well documented that activation of the mitochondrial pathway promotes mitochondrial fragmentation and inhibition of mitochondrial fragmentation partly represses cell death. However, the mitochondrial events following activation of the cytosolic pathway are less understood. In this study, we treated Fas-activating antibody and found mitochondrial fragmentation without cell death in hippocampal primary neurons and HT-22 cell lines. Fas antibody treatment, in fact, promoted rapid activation of caspase-8, while executioner caspase-3 activation was not observed. Furthermore, blockage of caspase-8 efficiently prevented Fas antibody-induced mitochondrial fragmentation. These results suggest that the cytosolic pathway induced by death receptor activation promotes caspase-8-dependent mitochondrial fission. The Korean Society for Brain and Neural Science 2015-09 2015-09-17 /pmc/articles/PMC4580749/ /pubmed/26412971 http://dx.doi.org/10.5607/en.2015.24.3.219 Text en Copyright © Experimental Neurobiology 2015. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Cho, Hyo Min
Sun, Woong
Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons
title Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons
title_full Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons
title_fullStr Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons
title_full_unstemmed Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons
title_short Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons
title_sort control of mitochondrial dynamics by fas-induced caspase-8 activation in hippocampal neurons
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580749/
https://www.ncbi.nlm.nih.gov/pubmed/26412971
http://dx.doi.org/10.5607/en.2015.24.3.219
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