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Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons
Cells undergo apoptosis mainly via two pathways-the mitochondrial pathway and the cytosolic pathway. It has been well documented that activation of the mitochondrial pathway promotes mitochondrial fragmentation and inhibition of mitochondrial fragmentation partly represses cell death. However, the m...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Brain and Neural Science
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580749/ https://www.ncbi.nlm.nih.gov/pubmed/26412971 http://dx.doi.org/10.5607/en.2015.24.3.219 |
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author | Cho, Hyo Min Sun, Woong |
author_facet | Cho, Hyo Min Sun, Woong |
author_sort | Cho, Hyo Min |
collection | PubMed |
description | Cells undergo apoptosis mainly via two pathways-the mitochondrial pathway and the cytosolic pathway. It has been well documented that activation of the mitochondrial pathway promotes mitochondrial fragmentation and inhibition of mitochondrial fragmentation partly represses cell death. However, the mitochondrial events following activation of the cytosolic pathway are less understood. In this study, we treated Fas-activating antibody and found mitochondrial fragmentation without cell death in hippocampal primary neurons and HT-22 cell lines. Fas antibody treatment, in fact, promoted rapid activation of caspase-8, while executioner caspase-3 activation was not observed. Furthermore, blockage of caspase-8 efficiently prevented Fas antibody-induced mitochondrial fragmentation. These results suggest that the cytosolic pathway induced by death receptor activation promotes caspase-8-dependent mitochondrial fission. |
format | Online Article Text |
id | pubmed-4580749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Korean Society for Brain and Neural Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45807492015-09-25 Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons Cho, Hyo Min Sun, Woong Exp Neurobiol Original Article Cells undergo apoptosis mainly via two pathways-the mitochondrial pathway and the cytosolic pathway. It has been well documented that activation of the mitochondrial pathway promotes mitochondrial fragmentation and inhibition of mitochondrial fragmentation partly represses cell death. However, the mitochondrial events following activation of the cytosolic pathway are less understood. In this study, we treated Fas-activating antibody and found mitochondrial fragmentation without cell death in hippocampal primary neurons and HT-22 cell lines. Fas antibody treatment, in fact, promoted rapid activation of caspase-8, while executioner caspase-3 activation was not observed. Furthermore, blockage of caspase-8 efficiently prevented Fas antibody-induced mitochondrial fragmentation. These results suggest that the cytosolic pathway induced by death receptor activation promotes caspase-8-dependent mitochondrial fission. The Korean Society for Brain and Neural Science 2015-09 2015-09-17 /pmc/articles/PMC4580749/ /pubmed/26412971 http://dx.doi.org/10.5607/en.2015.24.3.219 Text en Copyright © Experimental Neurobiology 2015. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cho, Hyo Min Sun, Woong Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons |
title | Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons |
title_full | Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons |
title_fullStr | Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons |
title_full_unstemmed | Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons |
title_short | Control of Mitochondrial Dynamics by Fas-induced Caspase-8 Activation in Hippocampal Neurons |
title_sort | control of mitochondrial dynamics by fas-induced caspase-8 activation in hippocampal neurons |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4580749/ https://www.ncbi.nlm.nih.gov/pubmed/26412971 http://dx.doi.org/10.5607/en.2015.24.3.219 |
work_keys_str_mv | AT chohyomin controlofmitochondrialdynamicsbyfasinducedcaspase8activationinhippocampalneurons AT sunwoong controlofmitochondrialdynamicsbyfasinducedcaspase8activationinhippocampalneurons |