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Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain

Tissue engineering scaffolds are often designed without appropriate consideration for the translational potential of the material. Solid scaffolds implanted into central nervous system (CNS) tissue to promote regeneration may require tissue resection to accommodate implantation. Or alternatively, th...

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Autores principales: Rivet, Christopher J, Zhou, Kun, Gilbert, Ryan J, Finkelstein, David I, Forsythe, John S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581123/
https://www.ncbi.nlm.nih.gov/pubmed/25996265
http://dx.doi.org/10.1080/21592535.2015.1005527
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author Rivet, Christopher J
Zhou, Kun
Gilbert, Ryan J
Finkelstein, David I
Forsythe, John S
author_facet Rivet, Christopher J
Zhou, Kun
Gilbert, Ryan J
Finkelstein, David I
Forsythe, John S
author_sort Rivet, Christopher J
collection PubMed
description Tissue engineering scaffolds are often designed without appropriate consideration for the translational potential of the material. Solid scaffolds implanted into central nervous system (CNS) tissue to promote regeneration may require tissue resection to accommodate implantation. Or alternatively, the solid scaffold may be cut or shaped to better fit an irregular injury geometry, but some features of the augmented scaffold may fail to integreate with surrounding tissue reducing regeneration potential. To create a biomaterial able to completely fill the irregular geometry of CNS injury and yet still provide sufficient cell migratory cues, an injectable, hybrid scaffold was created to present the physical architecture of electrospun fibers in an agarose/methylcellulose hydrogel. When injected into the rat striatum, infiltrating macrophages/microglia and resident astrocytes are able to locate the fibers and utilize their cues for migration into the hybrid matrix. Thus, hydrogels containing electrospun fibers may be an appropriate platform to encourage regeneration of the injured brain.
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spelling pubmed-45811232016-05-21 Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain Rivet, Christopher J Zhou, Kun Gilbert, Ryan J Finkelstein, David I Forsythe, John S Biomatter Short Communication Tissue engineering scaffolds are often designed without appropriate consideration for the translational potential of the material. Solid scaffolds implanted into central nervous system (CNS) tissue to promote regeneration may require tissue resection to accommodate implantation. Or alternatively, the solid scaffold may be cut or shaped to better fit an irregular injury geometry, but some features of the augmented scaffold may fail to integreate with surrounding tissue reducing regeneration potential. To create a biomaterial able to completely fill the irregular geometry of CNS injury and yet still provide sufficient cell migratory cues, an injectable, hybrid scaffold was created to present the physical architecture of electrospun fibers in an agarose/methylcellulose hydrogel. When injected into the rat striatum, infiltrating macrophages/microglia and resident astrocytes are able to locate the fibers and utilize their cues for migration into the hybrid matrix. Thus, hydrogels containing electrospun fibers may be an appropriate platform to encourage regeneration of the injured brain. Taylor & Francis 2015-05-21 /pmc/articles/PMC4581123/ /pubmed/25996265 http://dx.doi.org/10.1080/21592535.2015.1005527 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Short Communication
Rivet, Christopher J
Zhou, Kun
Gilbert, Ryan J
Finkelstein, David I
Forsythe, John S
Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain
title Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain
title_full Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain
title_fullStr Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain
title_full_unstemmed Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain
title_short Cell infiltration into a 3D electrospun fiber and hydrogel hybrid scaffold implanted in the brain
title_sort cell infiltration into a 3d electrospun fiber and hydrogel hybrid scaffold implanted in the brain
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581123/
https://www.ncbi.nlm.nih.gov/pubmed/25996265
http://dx.doi.org/10.1080/21592535.2015.1005527
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