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Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus
The suprachiasmatic nucleus plays an important role in generating circadian rhythms in mammals. The lateral habenular nucleus (LHb) is closely linked to this structure. Interestingly, the LHb shows a rhythmic firing rate in vivo and in vitro, and sustained oscillation of rhythmic genes in vitro. How...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581166/ https://www.ncbi.nlm.nih.gov/pubmed/26213916 http://dx.doi.org/10.3390/ijms160816740 |
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author | Zhao, Zhigong Xu, Haiyan Liu, Yongmao Mu, Li Xiao, Jinyu Zhao, Hua |
author_facet | Zhao, Zhigong Xu, Haiyan Liu, Yongmao Mu, Li Xiao, Jinyu Zhao, Hua |
author_sort | Zhao, Zhigong |
collection | PubMed |
description | The suprachiasmatic nucleus plays an important role in generating circadian rhythms in mammals. The lateral habenular nucleus (LHb) is closely linked to this structure. Interestingly, the LHb shows a rhythmic firing rate in vivo and in vitro, and sustained oscillation of rhythmic genes in vitro. However, under the in vivo condition, whether rhythmic gene expression in the LHb has circadian rhythms remains unknown. In this study, we examined LHb tissue in rats to determine Period2 (Per2) gene and protein expression at six zeitgeber time points (ZT2, ZT6, ZT10, ZT14, ZT18, and ZT22) in a 12-h light and 12-h dark (LD) environment. We found that in the LD environment, Per2 gene expression and PER2 protein levels in the LHb were higher in the day and lower in the night, showing periodic oscillation, with a peak at ZT10 and a trough at ZT22 (Per2 mRNA) and ZT18 (PER2 protein). We conclude that Per2 expression and PER2 protein levels in the LHb have rhythmic oscillation in vivo. This study provides a basis for further study on the role of the LHb in the circadian rhythm system. |
format | Online Article Text |
id | pubmed-4581166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-45811662015-09-28 Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus Zhao, Zhigong Xu, Haiyan Liu, Yongmao Mu, Li Xiao, Jinyu Zhao, Hua Int J Mol Sci Article The suprachiasmatic nucleus plays an important role in generating circadian rhythms in mammals. The lateral habenular nucleus (LHb) is closely linked to this structure. Interestingly, the LHb shows a rhythmic firing rate in vivo and in vitro, and sustained oscillation of rhythmic genes in vitro. However, under the in vivo condition, whether rhythmic gene expression in the LHb has circadian rhythms remains unknown. In this study, we examined LHb tissue in rats to determine Period2 (Per2) gene and protein expression at six zeitgeber time points (ZT2, ZT6, ZT10, ZT14, ZT18, and ZT22) in a 12-h light and 12-h dark (LD) environment. We found that in the LD environment, Per2 gene expression and PER2 protein levels in the LHb were higher in the day and lower in the night, showing periodic oscillation, with a peak at ZT10 and a trough at ZT22 (Per2 mRNA) and ZT18 (PER2 protein). We conclude that Per2 expression and PER2 protein levels in the LHb have rhythmic oscillation in vivo. This study provides a basis for further study on the role of the LHb in the circadian rhythm system. MDPI 2015-07-23 /pmc/articles/PMC4581166/ /pubmed/26213916 http://dx.doi.org/10.3390/ijms160816740 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Zhigong Xu, Haiyan Liu, Yongmao Mu, Li Xiao, Jinyu Zhao, Hua Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus |
title | Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus |
title_full | Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus |
title_fullStr | Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus |
title_full_unstemmed | Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus |
title_short | Diurnal Expression of the Per2 Gene and Protein in the Lateral Habenular Nucleus |
title_sort | diurnal expression of the per2 gene and protein in the lateral habenular nucleus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581166/ https://www.ncbi.nlm.nih.gov/pubmed/26213916 http://dx.doi.org/10.3390/ijms160816740 |
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