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Differential DNA Methylation in Relation to Age and Health Risks of Obesity

The aim of this study was to evaluate whether genome-wide levels of DNA methylation are associated with age and the health risks of obesity (HRO); defined according to BMI categories as “Low HRO” (overweight and class 1 obesity) versus “High HRO” (class 2 and class 3 obesity). Anthropometric measure...

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Autores principales: Mansego, María Luisa, Milagro, Fermín I., Zulet, María Ángeles, Moreno-Aliaga, María J., Martínez, José Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581172/
https://www.ncbi.nlm.nih.gov/pubmed/26213922
http://dx.doi.org/10.3390/ijms160816816
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author Mansego, María Luisa
Milagro, Fermín I.
Zulet, María Ángeles
Moreno-Aliaga, María J.
Martínez, José Alfredo
author_facet Mansego, María Luisa
Milagro, Fermín I.
Zulet, María Ángeles
Moreno-Aliaga, María J.
Martínez, José Alfredo
author_sort Mansego, María Luisa
collection PubMed
description The aim of this study was to evaluate whether genome-wide levels of DNA methylation are associated with age and the health risks of obesity (HRO); defined according to BMI categories as “Low HRO” (overweight and class 1 obesity) versus “High HRO” (class 2 and class 3 obesity). Anthropometric measurements were assessed in a subsample of 48 volunteers from the Metabolic Syndrome Reduction in Navarra (RESMENA) study and 24 women from another independent study, Effects of Lipoic Acid and Eicosapentaenoic Acid in Human Obesity (OBEPALIP study). In the pooled population; the methylation levels of 55 CpG sites were significantly associated with age after Benjamini-Hochberg correction. In addition, DNA methylation of three CpG sites located in ELOVL2; HOXC4 and PI4KB were further negatively associated with their mRNA levels. Although no differentially methylated CpG sites were identified in relation to HRO after multiple testing correction; several nominally significant CpG sites were identified in genes related to insulin signaling; energy and lipid metabolism. Moreover, statistically significant associations between BMI or mRNA levels and two HRO-related CpG sites located in GPR133 and ITGB5 are reported. As a conclusion, these findings from two Spanish cohorts add knowledge about the important role of DNA methylation in the age-related regulation of gene expression. In addition; a relevant influence of age on DNA methylation in white blood cells was found, as well as, on a trend level, novel associations between DNA methylation and obesity.
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spelling pubmed-45811722015-09-28 Differential DNA Methylation in Relation to Age and Health Risks of Obesity Mansego, María Luisa Milagro, Fermín I. Zulet, María Ángeles Moreno-Aliaga, María J. Martínez, José Alfredo Int J Mol Sci Article The aim of this study was to evaluate whether genome-wide levels of DNA methylation are associated with age and the health risks of obesity (HRO); defined according to BMI categories as “Low HRO” (overweight and class 1 obesity) versus “High HRO” (class 2 and class 3 obesity). Anthropometric measurements were assessed in a subsample of 48 volunteers from the Metabolic Syndrome Reduction in Navarra (RESMENA) study and 24 women from another independent study, Effects of Lipoic Acid and Eicosapentaenoic Acid in Human Obesity (OBEPALIP study). In the pooled population; the methylation levels of 55 CpG sites were significantly associated with age after Benjamini-Hochberg correction. In addition, DNA methylation of three CpG sites located in ELOVL2; HOXC4 and PI4KB were further negatively associated with their mRNA levels. Although no differentially methylated CpG sites were identified in relation to HRO after multiple testing correction; several nominally significant CpG sites were identified in genes related to insulin signaling; energy and lipid metabolism. Moreover, statistically significant associations between BMI or mRNA levels and two HRO-related CpG sites located in GPR133 and ITGB5 are reported. As a conclusion, these findings from two Spanish cohorts add knowledge about the important role of DNA methylation in the age-related regulation of gene expression. In addition; a relevant influence of age on DNA methylation in white blood cells was found, as well as, on a trend level, novel associations between DNA methylation and obesity. MDPI 2015-07-24 /pmc/articles/PMC4581172/ /pubmed/26213922 http://dx.doi.org/10.3390/ijms160816816 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mansego, María Luisa
Milagro, Fermín I.
Zulet, María Ángeles
Moreno-Aliaga, María J.
Martínez, José Alfredo
Differential DNA Methylation in Relation to Age and Health Risks of Obesity
title Differential DNA Methylation in Relation to Age and Health Risks of Obesity
title_full Differential DNA Methylation in Relation to Age and Health Risks of Obesity
title_fullStr Differential DNA Methylation in Relation to Age and Health Risks of Obesity
title_full_unstemmed Differential DNA Methylation in Relation to Age and Health Risks of Obesity
title_short Differential DNA Methylation in Relation to Age and Health Risks of Obesity
title_sort differential dna methylation in relation to age and health risks of obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581172/
https://www.ncbi.nlm.nih.gov/pubmed/26213922
http://dx.doi.org/10.3390/ijms160816816
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