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miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2

Background: MiR-198 has been considered as an inhibitor of cell proliferation, invasion, migration and a promoter of apoptosis in most cancer cells, while its effect on non-cancer cells is poorly understood. Methods: The effect of miR-198 transfection on HaCaT cell proliferation was firstly detected...

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Autores principales: Wang, Jian, Dan, Guorong, Shangguan, Tao, Hao, Han, Tang, Ran, Peng, Kaige, Zhao, Jiqing, Sun, Huiqin, Zou, Zhongmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581182/
https://www.ncbi.nlm.nih.gov/pubmed/26225959
http://dx.doi.org/10.3390/ijms160817018
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author Wang, Jian
Dan, Guorong
Shangguan, Tao
Hao, Han
Tang, Ran
Peng, Kaige
Zhao, Jiqing
Sun, Huiqin
Zou, Zhongmin
author_facet Wang, Jian
Dan, Guorong
Shangguan, Tao
Hao, Han
Tang, Ran
Peng, Kaige
Zhao, Jiqing
Sun, Huiqin
Zou, Zhongmin
author_sort Wang, Jian
collection PubMed
description Background: MiR-198 has been considered as an inhibitor of cell proliferation, invasion, migration and a promoter of apoptosis in most cancer cells, while its effect on non-cancer cells is poorly understood. Methods: The effect of miR-198 transfection on HaCaT cell proliferation was firstly detected using Cell Count Kit-8 and the cell cycle progression was analyzed by flow cytometry. Using bioinformatics analyses and luciferase assay, a new target of miR-198 was searched and identified. Then, the effect of the new target gene of miR-198 on cell proliferation and cell cycle was also detected. Results: Here we showed that miR-198 directly bound to the 3′-UTR of CCND2 mRNA, which was a key regulator in cell cycle progression. Overexpressed miR-198 repressed CCND2 expression at mRNA and protein levels and subsequently led to cell proliferation inhibition and cell cycle arrest in the G1 phase. Transfection ofSiCCND2 in HaCaT cells showed similar inhibitory effects on cell proliferation and cell cycle progression. Conclusion: In conclusion, we have identified that miR-198 inhibited HaCaT cell proliferation by directly targeting CCND2.
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spelling pubmed-45811822015-09-28 miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2 Wang, Jian Dan, Guorong Shangguan, Tao Hao, Han Tang, Ran Peng, Kaige Zhao, Jiqing Sun, Huiqin Zou, Zhongmin Int J Mol Sci Article Background: MiR-198 has been considered as an inhibitor of cell proliferation, invasion, migration and a promoter of apoptosis in most cancer cells, while its effect on non-cancer cells is poorly understood. Methods: The effect of miR-198 transfection on HaCaT cell proliferation was firstly detected using Cell Count Kit-8 and the cell cycle progression was analyzed by flow cytometry. Using bioinformatics analyses and luciferase assay, a new target of miR-198 was searched and identified. Then, the effect of the new target gene of miR-198 on cell proliferation and cell cycle was also detected. Results: Here we showed that miR-198 directly bound to the 3′-UTR of CCND2 mRNA, which was a key regulator in cell cycle progression. Overexpressed miR-198 repressed CCND2 expression at mRNA and protein levels and subsequently led to cell proliferation inhibition and cell cycle arrest in the G1 phase. Transfection ofSiCCND2 in HaCaT cells showed similar inhibitory effects on cell proliferation and cell cycle progression. Conclusion: In conclusion, we have identified that miR-198 inhibited HaCaT cell proliferation by directly targeting CCND2. MDPI 2015-07-27 /pmc/articles/PMC4581182/ /pubmed/26225959 http://dx.doi.org/10.3390/ijms160817018 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Jian
Dan, Guorong
Shangguan, Tao
Hao, Han
Tang, Ran
Peng, Kaige
Zhao, Jiqing
Sun, Huiqin
Zou, Zhongmin
miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2
title miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2
title_full miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2
title_fullStr miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2
title_full_unstemmed miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2
title_short miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2
title_sort mir-198 represses the proliferation of hacat cells by targeting cyclin d2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581182/
https://www.ncbi.nlm.nih.gov/pubmed/26225959
http://dx.doi.org/10.3390/ijms160817018
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