Cargando…

Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures

Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements in chicken limb bud-derived chondrogenic cells in micromass...

Descripción completa

Detalles Bibliográficos
Autores principales: Juhász, Tamás, Szentléleky, Eszter, Szűcs Somogyi, Csilla, Takács, Roland, Dobrosi, Nóra, Engler, Máté, Tamás, Andrea, Reglődi, Dóra, Zákány, Róza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581197/
https://www.ncbi.nlm.nih.gov/pubmed/26230691
http://dx.doi.org/10.3390/ijms160817344
_version_ 1782391515859910656
author Juhász, Tamás
Szentléleky, Eszter
Szűcs Somogyi, Csilla
Takács, Roland
Dobrosi, Nóra
Engler, Máté
Tamás, Andrea
Reglődi, Dóra
Zákány, Róza
author_facet Juhász, Tamás
Szentléleky, Eszter
Szűcs Somogyi, Csilla
Takács, Roland
Dobrosi, Nóra
Engler, Máté
Tamás, Andrea
Reglődi, Dóra
Zákány, Róza
author_sort Juhász, Tamás
collection PubMed
description Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements in chicken limb bud-derived chondrogenic cells in micromass cell cultures. Since no data can be found if PACAP signaling is playing any role during mechanical stress in any tissues, we aimed to investigate its contribution in mechanotransduction during chondrogenesis. Expressions of the mRNAs of PACAP and its major receptor, PAC1 increased, while that of other receptors, VPAC1, VPAC2 decreased upon mechanical stimulus. Mechanical load enhanced the expression of collagen type X, a marker of hypertrophic differentiation of chondrocytes and PACAP addition attenuated this elevation. Moreover, exogenous PACAP also prevented the mechanical load evoked activation of hedgehog signaling: protein levels of Sonic and Indian Hedgehogs and Gli1 transcription factor were lowered while expressions of Gli2 and Gli3 were elevated by PACAP application during mechanical load. Our results suggest that mechanical load activates PACAP signaling and exogenous PACAP acts against the hypertrophy inducing effect of mechanical load.
format Online
Article
Text
id pubmed-4581197
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-45811972015-09-28 Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures Juhász, Tamás Szentléleky, Eszter Szűcs Somogyi, Csilla Takács, Roland Dobrosi, Nóra Engler, Máté Tamás, Andrea Reglődi, Dóra Zákány, Róza Int J Mol Sci Article Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurohormone exerting protective function during various stress conditions either in mature or developing tissues. Previously we proved the presence of PACAP signaling elements in chicken limb bud-derived chondrogenic cells in micromass cell cultures. Since no data can be found if PACAP signaling is playing any role during mechanical stress in any tissues, we aimed to investigate its contribution in mechanotransduction during chondrogenesis. Expressions of the mRNAs of PACAP and its major receptor, PAC1 increased, while that of other receptors, VPAC1, VPAC2 decreased upon mechanical stimulus. Mechanical load enhanced the expression of collagen type X, a marker of hypertrophic differentiation of chondrocytes and PACAP addition attenuated this elevation. Moreover, exogenous PACAP also prevented the mechanical load evoked activation of hedgehog signaling: protein levels of Sonic and Indian Hedgehogs and Gli1 transcription factor were lowered while expressions of Gli2 and Gli3 were elevated by PACAP application during mechanical load. Our results suggest that mechanical load activates PACAP signaling and exogenous PACAP acts against the hypertrophy inducing effect of mechanical load. MDPI 2015-07-29 /pmc/articles/PMC4581197/ /pubmed/26230691 http://dx.doi.org/10.3390/ijms160817344 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Juhász, Tamás
Szentléleky, Eszter
Szűcs Somogyi, Csilla
Takács, Roland
Dobrosi, Nóra
Engler, Máté
Tamás, Andrea
Reglődi, Dóra
Zákány, Róza
Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures
title Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures
title_full Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures
title_fullStr Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures
title_full_unstemmed Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures
title_short Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures
title_sort pituitary adenylate cyclase activating polypeptide (pacap) pathway is induced by mechanical load and reduces the activity of hedgehog signaling in chondrogenic micromass cell cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581197/
https://www.ncbi.nlm.nih.gov/pubmed/26230691
http://dx.doi.org/10.3390/ijms160817344
work_keys_str_mv AT juhasztamas pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures
AT szentlelekyeszter pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures
AT szucssomogyicsilla pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures
AT takacsroland pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures
AT dobrosinora pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures
AT englermate pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures
AT tamasandrea pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures
AT reglodidora pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures
AT zakanyroza pituitaryadenylatecyclaseactivatingpolypeptidepacappathwayisinducedbymechanicalloadandreducestheactivityofhedgehogsignalinginchondrogenicmicromasscellcultures