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Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults

Maternal nutrition can affect development, leading to long-term effects on the health of offspring. The most common outcome is programmed hypertension. We examined whether alterations in renal transcriptome are responsible for generating programmed hypertension among four different models using next...

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Autores principales: Tain, You-Lin, Hsu, Chien-Ning, Chan, Julie Y. H., Huang, Li-Tung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581224/
https://www.ncbi.nlm.nih.gov/pubmed/26247937
http://dx.doi.org/10.3390/ijms160817826
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author Tain, You-Lin
Hsu, Chien-Ning
Chan, Julie Y. H.
Huang, Li-Tung
author_facet Tain, You-Lin
Hsu, Chien-Ning
Chan, Julie Y. H.
Huang, Li-Tung
author_sort Tain, You-Lin
collection PubMed
description Maternal nutrition can affect development, leading to long-term effects on the health of offspring. The most common outcome is programmed hypertension. We examined whether alterations in renal transcriptome are responsible for generating programmed hypertension among four different models using next-generation RNA sequencing (NGS) technology. Pregnant Sprague-Dawley rats received 50% caloric restriction (CR), intraperitoneal injection of 45 mg/kg streptozotocin, 60% high-fructose (HF) diet, or 1% NaCl in drinking water to conduct CR, diabetes, HF, or high-salt models, respectively. All four models induced programmed hypertension in adult male offspring. We observed 16 shared genes in a two-week-old kidney among four different models. The identified differential expressed genes (DEGs) that are related to the regulation of blood pressure included Adrb3, Alb, Apoe, Calca, Kng1, Adm2, Guca2b, Hba2, Hba-a2, and Ppara. The peroxisome proliferator-activated receptor (PPAR) signaling pathway and glutathione metabolism pathway were shared by the CR, diabetes, and HF models. Conclusively, a variety of maternal nutritional insults induced the same phenotype—programmed hypertension with differential alterations of renal transcriptome in adult male offspring. The roles of DEGs identified by the NGS in this study deserve further clarification to develop ideal maternal dietary interventions and thus spare the next generations from the burden of hypertension.
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spelling pubmed-45812242015-09-28 Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults Tain, You-Lin Hsu, Chien-Ning Chan, Julie Y. H. Huang, Li-Tung Int J Mol Sci Article Maternal nutrition can affect development, leading to long-term effects on the health of offspring. The most common outcome is programmed hypertension. We examined whether alterations in renal transcriptome are responsible for generating programmed hypertension among four different models using next-generation RNA sequencing (NGS) technology. Pregnant Sprague-Dawley rats received 50% caloric restriction (CR), intraperitoneal injection of 45 mg/kg streptozotocin, 60% high-fructose (HF) diet, or 1% NaCl in drinking water to conduct CR, diabetes, HF, or high-salt models, respectively. All four models induced programmed hypertension in adult male offspring. We observed 16 shared genes in a two-week-old kidney among four different models. The identified differential expressed genes (DEGs) that are related to the regulation of blood pressure included Adrb3, Alb, Apoe, Calca, Kng1, Adm2, Guca2b, Hba2, Hba-a2, and Ppara. The peroxisome proliferator-activated receptor (PPAR) signaling pathway and glutathione metabolism pathway were shared by the CR, diabetes, and HF models. Conclusively, a variety of maternal nutritional insults induced the same phenotype—programmed hypertension with differential alterations of renal transcriptome in adult male offspring. The roles of DEGs identified by the NGS in this study deserve further clarification to develop ideal maternal dietary interventions and thus spare the next generations from the burden of hypertension. MDPI 2015-08-03 /pmc/articles/PMC4581224/ /pubmed/26247937 http://dx.doi.org/10.3390/ijms160817826 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tain, You-Lin
Hsu, Chien-Ning
Chan, Julie Y. H.
Huang, Li-Tung
Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults
title Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults
title_full Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults
title_fullStr Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults
title_full_unstemmed Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults
title_short Renal Transcriptome Analysis of Programmed Hypertension Induced by Maternal Nutritional Insults
title_sort renal transcriptome analysis of programmed hypertension induced by maternal nutritional insults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581224/
https://www.ncbi.nlm.nih.gov/pubmed/26247937
http://dx.doi.org/10.3390/ijms160817826
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