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Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of the above...
Autores principales: | Sato, Masahiro, Koriyama, Miyu, Watanabe, Satoshi, Ohtsuka, Masato, Sakurai, Takayuki, Inada, Emi, Saitoh, Issei, Nakamura, Shingo, Miyoshi, Kazuchika |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581225/ https://www.ncbi.nlm.nih.gov/pubmed/26247938 http://dx.doi.org/10.3390/ijms160817838 |
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