Cargando…
Inhibition of NF-κB in Tumor Cells Exacerbates Immune Cell Activation Following Photodynamic Therapy
Although photodynamic therapy (PDT) yields very good outcomes in numerous types of superficial solid cancers, some tumors respond suboptimally to PDT. Novel treatment strategies are therefore needed to enhance the efficacy in these therapy-resistant tumors. One of these strategies is to combine PDT...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581334/ https://www.ncbi.nlm.nih.gov/pubmed/26307977 http://dx.doi.org/10.3390/ijms160819960 |
_version_ | 1782391546827505664 |
---|---|
author | Broekgaarden, Mans Kos, Milan Jurg, Freek A. van Beek, Adriaan A. van Gulik, Thomas M. Heger, Michal |
author_facet | Broekgaarden, Mans Kos, Milan Jurg, Freek A. van Beek, Adriaan A. van Gulik, Thomas M. Heger, Michal |
author_sort | Broekgaarden, Mans |
collection | PubMed |
description | Although photodynamic therapy (PDT) yields very good outcomes in numerous types of superficial solid cancers, some tumors respond suboptimally to PDT. Novel treatment strategies are therefore needed to enhance the efficacy in these therapy-resistant tumors. One of these strategies is to combine PDT with inhibitors of PDT-induced survival pathways. In this respect, the transcription factor nuclear factor κB (NF-κB) has been identified as a potential pharmacological target, albeit inhibition of NF-κB may concurrently dampen the subsequent anti-tumor immune response required for complete tumor eradication and abscopal effects. In contrast to these postulations, this study demonstrated that siRNA knockdown of NF-κB in murine breast carcinoma (EMT-6) cells increased survival signaling in these cells and exacerbated the inflammatory response in murine RAW 264.7 macrophages. These results suggest a pro-death and immunosuppressive role of NF-κB in PDT-treated cells that concurs with a hyperstimulated immune response in innate immune cells. |
format | Online Article Text |
id | pubmed-4581334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-45813342015-09-28 Inhibition of NF-κB in Tumor Cells Exacerbates Immune Cell Activation Following Photodynamic Therapy Broekgaarden, Mans Kos, Milan Jurg, Freek A. van Beek, Adriaan A. van Gulik, Thomas M. Heger, Michal Int J Mol Sci Article Although photodynamic therapy (PDT) yields very good outcomes in numerous types of superficial solid cancers, some tumors respond suboptimally to PDT. Novel treatment strategies are therefore needed to enhance the efficacy in these therapy-resistant tumors. One of these strategies is to combine PDT with inhibitors of PDT-induced survival pathways. In this respect, the transcription factor nuclear factor κB (NF-κB) has been identified as a potential pharmacological target, albeit inhibition of NF-κB may concurrently dampen the subsequent anti-tumor immune response required for complete tumor eradication and abscopal effects. In contrast to these postulations, this study demonstrated that siRNA knockdown of NF-κB in murine breast carcinoma (EMT-6) cells increased survival signaling in these cells and exacerbated the inflammatory response in murine RAW 264.7 macrophages. These results suggest a pro-death and immunosuppressive role of NF-κB in PDT-treated cells that concurs with a hyperstimulated immune response in innate immune cells. MDPI 2015-08-21 /pmc/articles/PMC4581334/ /pubmed/26307977 http://dx.doi.org/10.3390/ijms160819960 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Broekgaarden, Mans Kos, Milan Jurg, Freek A. van Beek, Adriaan A. van Gulik, Thomas M. Heger, Michal Inhibition of NF-κB in Tumor Cells Exacerbates Immune Cell Activation Following Photodynamic Therapy |
title | Inhibition of NF-κB in Tumor Cells Exacerbates Immune Cell Activation Following Photodynamic Therapy |
title_full | Inhibition of NF-κB in Tumor Cells Exacerbates Immune Cell Activation Following Photodynamic Therapy |
title_fullStr | Inhibition of NF-κB in Tumor Cells Exacerbates Immune Cell Activation Following Photodynamic Therapy |
title_full_unstemmed | Inhibition of NF-κB in Tumor Cells Exacerbates Immune Cell Activation Following Photodynamic Therapy |
title_short | Inhibition of NF-κB in Tumor Cells Exacerbates Immune Cell Activation Following Photodynamic Therapy |
title_sort | inhibition of nf-κb in tumor cells exacerbates immune cell activation following photodynamic therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581334/ https://www.ncbi.nlm.nih.gov/pubmed/26307977 http://dx.doi.org/10.3390/ijms160819960 |
work_keys_str_mv | AT broekgaardenmans inhibitionofnfkbintumorcellsexacerbatesimmunecellactivationfollowingphotodynamictherapy AT kosmilan inhibitionofnfkbintumorcellsexacerbatesimmunecellactivationfollowingphotodynamictherapy AT jurgfreeka inhibitionofnfkbintumorcellsexacerbatesimmunecellactivationfollowingphotodynamictherapy AT vanbeekadriaana inhibitionofnfkbintumorcellsexacerbatesimmunecellactivationfollowingphotodynamictherapy AT vangulikthomasm inhibitionofnfkbintumorcellsexacerbatesimmunecellactivationfollowingphotodynamictherapy AT hegermichal inhibitionofnfkbintumorcellsexacerbatesimmunecellactivationfollowingphotodynamictherapy |