Cargando…

Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice

Myostatin, a transforming growth factor-β family member, is a negative regulator of skeletal muscle development and growth. Piedmontese cattle breeds have a missense mutation, which results in a cysteine to tyrosine substitution in the mature myostatin protein (C313Y). This loss-of-function mutation...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Dezun, Gao, Pengfei, Qian, Lili, Wang, Qingqing, Cai, Chunbo, Jiang, Shengwang, Xiao, Gaojun, Cui, Wentao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581338/
https://www.ncbi.nlm.nih.gov/pubmed/26305245
http://dx.doi.org/10.3390/ijms160820020
_version_ 1782391547735572480
author Ma, Dezun
Gao, Pengfei
Qian, Lili
Wang, Qingqing
Cai, Chunbo
Jiang, Shengwang
Xiao, Gaojun
Cui, Wentao
author_facet Ma, Dezun
Gao, Pengfei
Qian, Lili
Wang, Qingqing
Cai, Chunbo
Jiang, Shengwang
Xiao, Gaojun
Cui, Wentao
author_sort Ma, Dezun
collection PubMed
description Myostatin, a transforming growth factor-β family member, is a negative regulator of skeletal muscle development and growth. Piedmontese cattle breeds have a missense mutation, which results in a cysteine to tyrosine substitution in the mature myostatin protein (C313Y). This loss-of-function mutation in myostatin results in a double-muscled phenotype in cattle. Myostatin propeptide is an inhibitor of myostatin activity and is considered a potential agent to stimulate muscle growth in livestock. In this study, we generated transgenic mice overexpressing porcine myostatin missense mutant (pmMS), C313Y, and wild-type porcine myostatin propeptide (ppMS), respectively, to examine their effects on muscle growth in mice. Enhanced muscle growth was observed in both pmMS and ppMS transgenic female mice and also in ppMS transgenic male mice. However, there was no enhanced muscle growth observed in pmMS transgenic male mice. To explore why there is such a big difference in muscle growth between pmMS and ppMS transgenic male mice, the expression level of androgen receptor (AR) mutant AR45 was measured by Western blot. Results indicated that AR45 expression significantly increased in pmMS transgenic male mice while it decreased dramatically in ppMS transgenic male mice. Our data demonstrate that both pmMS and ppMS act as myostatin inhibitors in the regulation of muscle growth, but the effect of pmMS in male mice is reversed by an increased AR45 expression. These results provide useful insight and basic theory to future studies on improving pork quality by genetically manipulating myostatin expression or by regulating myostatin activity.
format Online
Article
Text
id pubmed-4581338
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-45813382015-09-28 Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice Ma, Dezun Gao, Pengfei Qian, Lili Wang, Qingqing Cai, Chunbo Jiang, Shengwang Xiao, Gaojun Cui, Wentao Int J Mol Sci Article Myostatin, a transforming growth factor-β family member, is a negative regulator of skeletal muscle development and growth. Piedmontese cattle breeds have a missense mutation, which results in a cysteine to tyrosine substitution in the mature myostatin protein (C313Y). This loss-of-function mutation in myostatin results in a double-muscled phenotype in cattle. Myostatin propeptide is an inhibitor of myostatin activity and is considered a potential agent to stimulate muscle growth in livestock. In this study, we generated transgenic mice overexpressing porcine myostatin missense mutant (pmMS), C313Y, and wild-type porcine myostatin propeptide (ppMS), respectively, to examine their effects on muscle growth in mice. Enhanced muscle growth was observed in both pmMS and ppMS transgenic female mice and also in ppMS transgenic male mice. However, there was no enhanced muscle growth observed in pmMS transgenic male mice. To explore why there is such a big difference in muscle growth between pmMS and ppMS transgenic male mice, the expression level of androgen receptor (AR) mutant AR45 was measured by Western blot. Results indicated that AR45 expression significantly increased in pmMS transgenic male mice while it decreased dramatically in ppMS transgenic male mice. Our data demonstrate that both pmMS and ppMS act as myostatin inhibitors in the regulation of muscle growth, but the effect of pmMS in male mice is reversed by an increased AR45 expression. These results provide useful insight and basic theory to future studies on improving pork quality by genetically manipulating myostatin expression or by regulating myostatin activity. MDPI 2015-08-24 /pmc/articles/PMC4581338/ /pubmed/26305245 http://dx.doi.org/10.3390/ijms160820020 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Dezun
Gao, Pengfei
Qian, Lili
Wang, Qingqing
Cai, Chunbo
Jiang, Shengwang
Xiao, Gaojun
Cui, Wentao
Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice
title Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice
title_full Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice
title_fullStr Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice
title_full_unstemmed Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice
title_short Over-Expression of Porcine Myostatin Missense Mutant Leads to A Gender Difference in Skeletal Muscle Growth between Transgenic Male and Female Mice
title_sort over-expression of porcine myostatin missense mutant leads to a gender difference in skeletal muscle growth between transgenic male and female mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581338/
https://www.ncbi.nlm.nih.gov/pubmed/26305245
http://dx.doi.org/10.3390/ijms160820020
work_keys_str_mv AT madezun overexpressionofporcinemyostatinmissensemutantleadstoagenderdifferenceinskeletalmusclegrowthbetweentransgenicmaleandfemalemice
AT gaopengfei overexpressionofporcinemyostatinmissensemutantleadstoagenderdifferenceinskeletalmusclegrowthbetweentransgenicmaleandfemalemice
AT qianlili overexpressionofporcinemyostatinmissensemutantleadstoagenderdifferenceinskeletalmusclegrowthbetweentransgenicmaleandfemalemice
AT wangqingqing overexpressionofporcinemyostatinmissensemutantleadstoagenderdifferenceinskeletalmusclegrowthbetweentransgenicmaleandfemalemice
AT caichunbo overexpressionofporcinemyostatinmissensemutantleadstoagenderdifferenceinskeletalmusclegrowthbetweentransgenicmaleandfemalemice
AT jiangshengwang overexpressionofporcinemyostatinmissensemutantleadstoagenderdifferenceinskeletalmusclegrowthbetweentransgenicmaleandfemalemice
AT xiaogaojun overexpressionofporcinemyostatinmissensemutantleadstoagenderdifferenceinskeletalmusclegrowthbetweentransgenicmaleandfemalemice
AT cuiwentao overexpressionofporcinemyostatinmissensemutantleadstoagenderdifferenceinskeletalmusclegrowthbetweentransgenicmaleandfemalemice