MicroRNAs in urine are not biomarkers of multiple myeloma

BACKGROUND: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease. RESULTS: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays fo...

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Autores principales: Sedlaříková, Lenka, Bešše, Lenka, Novosadová, Soňa, Kubaczková, Veronika, Radová, Lenka, Staník, Michal, Krejčí, Marta, Hájek, Roman, Ševčíková, Sabina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581458/
https://www.ncbi.nlm.nih.gov/pubmed/26399230
http://dx.doi.org/10.1186/s12952-015-0035-7
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author Sedlaříková, Lenka
Bešše, Lenka
Novosadová, Soňa
Kubaczková, Veronika
Radová, Lenka
Staník, Michal
Krejčí, Marta
Hájek, Roman
Ševčíková, Sabina
author_facet Sedlaříková, Lenka
Bešše, Lenka
Novosadová, Soňa
Kubaczková, Veronika
Radová, Lenka
Staník, Michal
Krejčí, Marta
Hájek, Roman
Ševčíková, Sabina
author_sort Sedlaříková, Lenka
collection PubMed
description BACKGROUND: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease. RESULTS: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance. CONCLUSIONS: Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.
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spelling pubmed-45814582015-09-25 MicroRNAs in urine are not biomarkers of multiple myeloma Sedlaříková, Lenka Bešše, Lenka Novosadová, Soňa Kubaczková, Veronika Radová, Lenka Staník, Michal Krejčí, Marta Hájek, Roman Ševčíková, Sabina J Negat Results Biomed Brief Report BACKGROUND: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease. RESULTS: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance. CONCLUSIONS: Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma. BioMed Central 2015-09-23 /pmc/articles/PMC4581458/ /pubmed/26399230 http://dx.doi.org/10.1186/s12952-015-0035-7 Text en © Sedlaříková et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Brief Report
Sedlaříková, Lenka
Bešše, Lenka
Novosadová, Soňa
Kubaczková, Veronika
Radová, Lenka
Staník, Michal
Krejčí, Marta
Hájek, Roman
Ševčíková, Sabina
MicroRNAs in urine are not biomarkers of multiple myeloma
title MicroRNAs in urine are not biomarkers of multiple myeloma
title_full MicroRNAs in urine are not biomarkers of multiple myeloma
title_fullStr MicroRNAs in urine are not biomarkers of multiple myeloma
title_full_unstemmed MicroRNAs in urine are not biomarkers of multiple myeloma
title_short MicroRNAs in urine are not biomarkers of multiple myeloma
title_sort micrornas in urine are not biomarkers of multiple myeloma
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4581458/
https://www.ncbi.nlm.nih.gov/pubmed/26399230
http://dx.doi.org/10.1186/s12952-015-0035-7
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